Nyamavhuvhu 26, 2015 / Kyushu University / Scientific Reports

Chinyorwa/Wu Tingyao

Boka rekutsvagisa raKuniyoshi Shimizu, purofesa anobatana paInstitute of Agricultural Sciences yeKyushu University muJapan, akasimbisa kuti 31 triterpenoids yakaparadzaniswa nemuviri weGanoderma inobereka michero inodzivirira neuraminidase yevashanu furuwenza A mavhairasi kune akasiyana madhigirii, pakati pawo pane maviri. triterpenoids yakatokodzera kuvandudzwa semishonga inorwisa furuwenza.Mhedzisiro yetsvagiridzo yakaburitswa mu "Scientific Reports" pasi pe "Nature" boka rekuburitsa pakupera kwaNyamavhuvhu 2015.

Neuraminidase imwe yemapuroteni maviri anobuda pamusoro pehutachiona hwefuruwenza A.Utachiona hwefuruwenza hunosvika zana remaprotease aya.Kana hutachiona hwapinda musero huchishandisa zvinhu zviri musero kudzokorora twuutachiona hutsva, neuraminidase inodiwa kuti twumavhairasi hutsva hutyoke kubva musero huchiwedzera kutapurira mamwe maseru.Naizvozvo, apo neuraminidase inorasikirwa nebasa rayo, utachiona hutsva huchavharirwa muchitokisi uye hahugoni kutiza, kutyisidzira kumuridzi kuchaderedzwa, uye hosha yacho inogona kudzorwa.Iyo inowanzoshandiswa oseltamivir (Tamiflu) mumakiriniki maitiro ndeyekushandisa musimboti uyu kudzivirira kupararira nekupararira kwehutachiona.

Zvinoenderana netsvakiridzo yakaitwa naKuniyoshi Shimizu, pakusangana kwe200 μM, aya maGanoderma triterpenoids akavharisa basa reH1N1, H5N1, H7N9 uye maviri anodzivirira mutant strains NA (H1N1, N295S) uye NA (H3N2, E119V) kune akasiyana madhigirii.Pamusoro pese, inhibitory effect pane neuraminidase yeN1 type (kunyanya H5N1) ndiyo yakanakisisa, uye inhibitory effect pane neuraminidase yeH7N9 ndiyo yakaipisisa.Pakati peiyi triterpenoids, ganoderic acid TQ uye ganoderic acid TR yakaratidza mazinga akakwirira ekudzivisa, uye migumisiro yezvikamu zviviri izvi zvakabva ku55.4% kusvika ku96.5% inhibition kune zvakasiyana NA subtypes.

Kumwezve ongororo yehukama hwekuita-chiito cheiyi triterpenoids yakaratidza kuti triterpenoids, ine inhibitory zvirinani paN1 neuraminidase, ine chimiro chikuru che "tetracyclic triterpenoids ine maviri maviri bond, bazi seboka recarboxylic, uye oxygen- ine boka panzvimbo yeR5” (Kumusana A pamufananidzo uri pazasi).Kana iyo huru chimiro chiri mamwe maviri (Mushure B uye C mumufananidzo uri pazasi), mhedzisiro ichava yakaipa.

(Kwakabva/Sci Rep. 2015 Aug 26; 5:13194.)

Mu silico docking inoshandiswa kutevedzera kupindirana kweganoderic acids (TQ uye TR) uye neuraminidases (H1N1 uye H5N1).Somugumisiro, zvakaonekwa kuti zvose ganoderic acids uye Tamiflu vakakwanisa kusunga zvakananga kune nzvimbo inoshandiswa yeuraminidase.Iyi nzvimbo inoshanda inoumbwa neamino acid akati wandei.Ganoderma acids TQ uye TR ichasunga kune maviri amino acid akasara Arg292 uye Glu119.Tamiflu ine imwe sarudzo asi inogonawo kuita kuti neuraminidase isashande.

Zvichienzaniswa nekudzivisa mamwe mapuroteni pahutachiona hwefuruwenza (seprotein yeM2, iyo inovhura goko rehutachiona panguva iyo hutachiona hunosunga kune iyo inotakura sero uye inotumira hutachiona hwemajini musero), neuraminidase inhibitors ikozvino inozivikanwa se inoshanda uye shoma. resistant furuwenza mishonga.Nokudaro, vatsvakurudzi vanotenda kuti ganoderic acids TQ uye TR, iyo yakafanana asi isina kufanana mukugadzirisa kweTamiflu, ine mukana wekushandiswa sechizvarwa chitsva chemishonga inorwisa furuwenza kana zvinyorwa zvekugadzira.

Zvisinei, pane zvinodiwa kuti mushonga ushandiswe semushonga unorwisa furuwenza, kureva kuti, mushonga wacho unofanira kunyatsodzivisa kuberekana kwehutachiwana pasina kukuvadza masero ane utachiona.Nekudaro, mukuyedza kumaseru ane hutachiona hwehupenyu uye kenza yemazamu cell mitsara (MCF-7), zvakaonekwa kuti apo vaongorori vakashandisa aya marudzi maviri eganoderic acids chete, vaive nekusahadzika nezve yakakwira cytotoxicity, asi vakawanawo imwe mhando. yeGanoderma triterpenoid, ganoderol B, ine inhibitory effect paH5N1 (asi inhibitory effect haina kunaka), asi haisi cytotoxic.Nokudaro, vatsvakurudzi vanotenda kuti nzira yekuvandudza kuchengetedzwa kweganoderic acids TQ uye TR kuburikidza nekushandurwa kwechimiro chemakemikari vachiri kuchengetedza inhibition yavo yeuraminidase chiitiko inofanira kunyatsotariswa.

[Kwakabva] Zhu Q, et al.Inhibition ye neuraminidase neGanoderma triterpenoids uye zvinorehwa neuraminidase inhibitor design.Sci Rep. 2015 Aug 26;5:13194.doi: 10.1038/srep13194.

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Nezve munyori/ Ms. Wu Tingyao
Wu Tingyao anga achizivisa nezvekutanga-ruoko ruzivo rweGanoderma kubvira 1999. Ndiye munyori weKuporesa neGanoderma(yakabudiswa muThe People's Medical Publishing House muna Kubvumbi 2017).
 
★ Chinyorwa ichi chakatsikiswa pasi pemvumo yakasarudzika yemunyori.★ Mabhuku ari pamusoro haagone kutsikiswa, kutorwa kana kushandiswa nedzimwe nzira pasina mvumo yemunyori.★ Nekutyorwa kwechirevo chiri pamusoro, munyori achatevera mabasa akakodzera emutemo.★ Manyoro ekutanga echinyorwa ichi akanyorwa neChinese naWu Tingyao uye akashandurirwa muchiRungu naAlfred Liu.Kana paine mutsauko pakati peshanduro (yeChirungu) neyekutanga (Chinese), iyo yekutanga Chinese ichakunda.Kana vaverengi vaine mibvunzo, tapota taura nemunyori wemabviro, Ms. Wu Tingyao.