Lub Yim Hli 26, 2015 / Kyushu University / Daim Ntawv Qhia Txog Kev Tshawb Fawb

Text/Wu Tingyao

Pab pawg tshawb fawb ntawm Kuniyoshi Shimizu, tus kws tshaj lij ntawm lub koom haum ntawm Kev Tshawb Fawb Txog Kev Ua Liaj Ua Teb ntawm Kyushu University hauv Nyij Pooj, tau lees paub tias 31 triterpenoids cais tawm ntawm lub cev txiv hmab txiv ntoo ntawm Ganoderma inhibit neuraminidase ntawm tsib tus kab mob khaub thuas A mus rau qib sib txawv, ntawm cov uas muaj ob. triterpenoids txawm tsim nyog rau kev loj hlob raws li tshuaj tiv thaiv kab mob khaub thuas.Cov txiaj ntsig kev tshawb fawb tau luam tawm hauv "Cov Ntawv Qhia Txog Kev Tshawb Fawb" hauv qab "Nature" pawg tshaj tawm thaum kawg lub Yim Hli 2015.

Neuraminidase yog ib qho ntawm ob cov proteins uas tshwm sim rau ntawm tus kab mob khaub thuas A.Txhua tus kab mob khaub thuas muaj txog ib puas ntawm cov proteases.Thaum tus kab mob nkag mus rau hauv lub xovtooj thiab siv cov khoom siv hauv lub cell kom rov ua cov kab mob tshiab, neuraminidase yog qhov xav tau rau cov kab mob tshiab kom tawg tawm ntawm lub cell thiab kis mus rau lwm lub hlwb.Yog li ntawd, thaum neuraminidase poob nws txoj haujlwm, tus kab mob tshiab yuav raug kaw hauv lub xov tooj ntawm tes thiab tsis tuaj yeem khiav tawm, kev hem thawj rau tus tswv yuav raug txo, thiab tus kab mob tuaj yeem tswj tau.Feem ntau siv oseltamivir (Tamiflu) hauv kev kho mob yog siv txoj cai no los tiv thaiv kev loj hlob thiab kis tus kab mob.

Raws li kev tshawb fawb los ntawm Kuniyoshi Shimizu, ntawm qhov concentration ntawm 200 μM, cov Ganoderma triterpenoids inhibited cov haujlwm ntawm H1N1, H5N1, H7N9 thiab ob hom kab mob resistant NA (H1N1, N295S) thiab NA (H3N2, E119V) mus rau qhov sib txawv.Ntawm tag nrho, cov nyhuv inhibitory ntawm neuraminidase ntawm N1 hom (tshwj xeeb tshaj yog H5N1) yog qhov zoo tshaj plaws, thiab cov nyhuv inhibitory ntawm neuraminidase ntawm H7N9 yog qhov phem tshaj.Ntawm cov triterpenoids no, ganoderic acid TQ thiab ganoderic acid TR tau pom qhov siab tshaj plaws ntawm inhibition, thiab cov teebmeem ntawm ob lub tebchaw no txawv ntawm 55.4% mus rau 96.5% inhibition rau ntau hom NA subtypes.

Kev tshuaj xyuas ntxiv ntawm cov qauv-kev sib raug zoo ntawm cov triterpenoids no tau qhia tias triterpenoids, uas muaj cov nyhuv inhibitory zoo dua ntawm N1 neuraminidase, muaj cov qauv tseem ceeb ntawm "tetracyclic triterpenoids nrog ob daim ntawv cog lus ob, ceg ua ib pawg carboxylic, thiab cov pa oxygen- muaj cov pab pawg ntawm R5 qhov chaw” (Backbone A hauv daim duab hauv qab).Yog tias cov qauv tseem ceeb yog ob qho tib si (Backbone B thiab C hauv daim duab hauv qab no), cov nyhuv yuav tsis zoo.

(Source/Sci Rep. 2015 Aug 26; 5:13194.)

Hauv silico docking yog siv los simulate kev sib cuam tshuam ntawm ganoderic acids (TQ thiab TR) thiab neuraminidases (H1N1 thiab H5N1).Raws li qhov tshwm sim, nws tau pom tias ob qho tib si ganoderic acids thiab Tamiflu tuaj yeem txuas ncaj qha mus rau thaj chaw nquag ntawm neuraminidase.Qhov chaw ua haujlwm no yog tsim los ntawm ntau cov amino acid residues.Ganoderma acids TQ thiab TR yuav khi rau ob amino acid residues Arg292 thiab Glu119.Tamiflu muaj lwm txoj kev xaiv tab sis kuj tuaj yeem ua rau neuraminidase tsis zoo.

Piv nrog rau inhibiting lwm cov proteins ntawm tus kab mob khaub thuas (xws li M2 protein, uas qhib lub plhaub tus kab mob thaum lub sij hawm tus kab mob khi rau tus tswv tsev cell thiab xa cov kab mob mus rau lub cell), neuraminidase inhibitors tam sim no lees paub tias zoo thiab tsawg dua. tshuaj tiv thaiv kab mob khaub thuas.Yog li ntawd, cov kws tshawb fawb ntseeg tias ganoderic acids TQ thiab TR, uas zoo ib yam li tab sis tsis zoo ib yam hauv cov txheej txheem ntawm Tamiflu, muaj lub sijhawm los siv los ua cov tiam tshiab ntawm cov tshuaj tiv thaiv kab mob khaub thuas lossis cov ntaub ntawv tsim.

Txawm li cas los xij, muaj qhov yuav tsum tau ua ua ntej rau cov tshuaj siv los ua cov tshuaj tiv thaiv kab mob khaub thuas, uas yog, cov tshuaj yuav tsum muaj txiaj ntsig zoo inhibit qhov kev loj hlob ntawm tus kab mob yam tsis muaj kev puas tsuaj rau lub hlwb uas kis tus kab mob.Txawm li cas los xij, hauv kev sim ntawm cov hlwb uas muaj cov kab mob nyob thiab kab mob qog noj ntshav mis (MCF-7), nws tau pom tias thaum cov kws tshawb fawb siv ob hom ganoderic acids ib leeg, lawv muaj kev tsis ntseeg txog cytotoxicity siab, tab sis lawv kuj pom lwm yam. ntawm Ganoderma triterpenoid, ganoderol B, muaj cov nyhuv inhibitory ntawm H5N1 (tab sis cov nyhuv inhibitory tsis zoo), tab sis nws tsis yog cytotoxic.Yog li, cov kws tshawb fawb ntseeg tias yuav ua li cas txhawm rau txhim kho kev nyab xeeb ntawm ganoderic acids TQ thiab TR los ntawm kev hloov kho cov qauv tshuaj thaum tseem khaws lawv cov inhibition ntawm neuraminidase kev ua haujlwm yuav tsum tau ua tib zoo xav.

[Source] Zhu Q, et al.Inhibition ntawm neuraminidase los ntawm Ganoderma triterpenoids thiab cuam tshuam rau neuraminidase inhibitor tsim.Sci Rep. 26 Aug 2015; 5:13194.doi: 10.1038/srep13194.

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Hais txog tus sau/ Ms. Wu Tingyao
Wu Tingyao tau tshaj tawm cov ntaub ntawv Ganoderma thawj zaug txij li xyoo 1999. Nws yog tus sau ntawmKho nrog Ganoderma(tshaj tawm hauv Lub Tsev Kho Mob Tib Neeg Lub Plaub Hlis 2017).
 
★ Kab lus no tau luam tawm raws li kev tso cai tshwj xeeb ntawm tus sau.★ Cov haujlwm saum toj no tsis tuaj yeem muab luam tawm, txiav tawm lossis siv lwm txoj hauv kev yam tsis muaj kev tso cai los ntawm tus sau.★ Rau kev ua txhaum ntawm cov lus hais saum toj no, tus sau yuav ua raws li cov luag haujlwm raug cai.★ Cov ntawv qub ntawm kab lus no tau sau ua lus Suav los ntawm Wu Tingyao thiab txhais ua lus Askiv los ntawm Alfred Liu.Yog tias muaj qhov tsis sib xws ntawm kev txhais lus (English) thiab tus thawj (Suav), tus thawj Suav yuav yeej.Yog tias cov nyeem muaj lus nug, thov hu rau tus thawj kws sau ntawv, Ms. Wu Tingyao.