26 Kanama 2015 / Kaminuza ya Kyushu / Raporo yubumenyi

Inyandiko / Wu Tingyao

Itsinda ry’ubushakashatsi rya Kuniyoshi Shimizu, umwarimu wungirije mu kigo cy’ubumenyi bw’ubuhinzi muri kaminuza ya Kyushu mu Buyapani, ryemeje ko triterpenoide 31 zitandukanijwe n’umubiri wera imbuto za Ganoderma zibuza neuraminidase ya virusi eshanu z’ibicurane A ku buryo butandukanye, muri zo hakaba harimo ebyiri triterpenoide niyo ibereye iterambere nkibiyobyabwenge birwanya ibicurane.Ibisubizo byubushakashatsi byatangajwe muri "Raporo yubumenyi" munsi yitsinda ryandika "Kamere" mu mpera za Kanama 2015.

Neuraminidase ni imwe muri poroteyine ebyiri zigaragara hejuru ya virusi ya grippe A.Buri virusi ya grippe ifite proteine ​​zigera ku ijana.Iyo virusi yibasiye ingirabuzimafatizo kandi igakoresha ibikoresho biri mu ngirabuzimafatizo kugira ngo yigane uduce duto twa virusi, hakenerwa neuraminidase kugira ngo virusi nshya zive mu kagari kandi zanduze izindi selile.Kubwibyo, iyo neuraminidase itakaje ibikorwa byayo, virusi nshya izafungirwa muri selire kandi ntishobora guhunga, iterabwoba ryabakiriye rizagabanuka, kandi indwara irashobora kugenzurwa.Oseltamivir (Tamiflu) ikoreshwa cyane mubikorwa byubuvuzi ni ugukoresha iri hame kugirango wirinde ikwirakwizwa rya virusi.

Nk’uko ubushakashatsi bwakozwe na Kuniyoshi Shimizu bubitangaza, ku gipimo cya 200 μM, aba Ganoderma triterpenoide yabujije ibikorwa bya H1N1, H5N1, H7N9 hamwe n’imiterere ibiri irwanya NA (H1N1, N295S) na NA (H3N2, E119V) ku buryo butandukanye.Muri rusange, ingaruka zo kubuza neuraminidase yubwoko bwa N1 (cyane cyane H5N1) ninziza, kandi ingaruka zo guhagarika kuri neuraminidase ya H7N9 nizo mbi cyane.Muri izi triterpenoide, acide ganoderic TQ na acide ganoderic TR yerekanaga urwego rwo hejuru rwo kubuza, kandi ingaruka zibi bice byombi kuva kuri 55.4% kugeza kuri 96.5% kubuza ubwoko butandukanye bwa NA.

Ubundi isesengura ryimiterere-yimikorere yibikorwa bya triterpenoide ryerekanye ko triterpenoide, ifite ingaruka nziza yo kubuza N1 neuraminidase, ifite imiterere nyamukuru ya "tetracyclic triterpenoide ifitanye isano ebyiri, ishami nkitsinda rya karubasi, na ogisijeni- ikubiyemo itsinda kurubuga R5 "(Umugongo A mumashusho hepfo).Niba imiterere nyamukuru nizindi ebyiri (Umugongo B na C mumashusho hepfo), ingaruka zizaba mbi.

(Inkomoko / Sci Rep. 2015 Kanama 26; 5: 13194.)

Muri silico docking ikoreshwa mu kwigana imikoranire ya acide ganoderic (TQ na TR) na neuraminidase (H1N1 na H5N1).Kubera iyo mpamvu, byagaragaye ko acide ganoderic na Tamiflu byashoboye guhuza neza na neza na neuraminidase.Aka gace gakora kagizwe nibisigisigi byinshi bya aside amine.Acide ya Ganoderma TQ na TR izahuza ibisigisigi bibiri bya aside amine Arg292 na Glu119.Tamiflu ifite ubundi buryo ariko irashobora kandi gutuma neuraminidase idakora neza.

Ugereranije no guhagarika izindi poroteyine kuri virusi ya grippe (nka poroteyine M2, ifungura igikonjo cya virusi muri iki gihe virusi ihuza ingirabuzimafatizo kandi ikohereza ingirabuzimafatizo za selile), inzitizi za neuraminidase kuri ubu zizwi ko zifite akamaro kandi nkeya imiti ivura ibicurane.Abashakashatsi rero bemeza ko acide ganoderic TQ na TR, bisa ariko bitameze nkuburyo bwa Tamiflu, bifite amahirwe yo gukoreshwa nkibisekuru bishya byibiyobyabwenge birwanya ibicurane cyangwa ibishushanyo mbonera.

Icyakora, hari icyangombwa kugirango imiti ikoreshwe nk'umuti urwanya ibicurane, ni ukuvuga ko imiti igomba kubuza neza iyororoka rya virusi itiriwe yangiza selile zanduye virusi.Icyakora, mu bushakashatsi bwakozwe ku ngirabuzimafatizo zanduye virusi nzima n'imirongo ya kanseri y'ibere (MCF-7), byagaragaye ko igihe abashakashatsi bakoreshaga ubwo bwoko bubiri bwa acide ganoderic bonyine, bashidikanyaga kuri cytotoxicite nyinshi, ariko bakabona n'ubundi bwoko ya Ganoderma triterpenoid, ganoderol B, igira ingaruka mbi kuri H5N1 (ariko ingaruka zo kubuza ni mbi), ariko ntabwo ari cytotoxic.Kubwibyo, abashakashatsi bemeza ko uburyo bwo kuzamura umutekano wa acide ganoderic TQ na TR binyuze muguhindura imiterere yimiti mugihe bagikomeza kubuza ibikorwa bya neuraminidase bigomba gutekerezwa neza.

[Inkomoko] Zhu Q, n'abandi.Kubuza neuraminidase na Ganoderma triterpenoids hamwe ningaruka zo gushushanya neuraminidase inhibitor.Sci Rep. 2015 Kanama 26; 5: 13194.doi: 10.1038 / srep13194.

IHEREZO

Ibyerekeye umwanditsi / Madamu Wu Tingyao
Wu Tingyao yagiye atanga amakuru ku makuru ya Ganoderma kuva mu 1999. Ni umwanditsi waGukiza hamwe na Ganoderma(byasohotse mu gitabo cy’ubuvuzi cy’abaturage muri Mata 2017).
 
★ Iyi ngingo yasohowe uruhushya rwihariye rwumwanditsi.Works Ibikorwa byavuzwe haruguru ntibishobora gusubirwamo, gucibwa cyangwa gukoreshwa mubundi buryo utabiherewe uburenganzira n'umwanditsi.★ Kurenga ku magambo yavuzwe haruguru, umwanditsi azakurikirana inshingano zemewe n'amategeko.Text Umwandiko wumwimerere wiyi ngingo wanditswe mu gishinwa na Wu Tingyao uhindurwa mu cyongereza na Alfred Liu.Niba hari itandukaniro riri hagati yubuhinduzi (icyongereza) numwimerere (igishinwa), igishinwa cyumwimerere kizatsinda.Niba abasomyi bafite ikibazo, nyamuneka hamagara umwanditsi wambere, Madamu Wu Tingyao.