ʻAukake 26, 2015 / Kyushu University / Hōʻike ʻepekema

Text/Wu Tingyao

Ua hōʻoia ka hui noiʻi o Kuniyoshi Shimizu, he kumu aʻoaʻo ma ka Institute of Agricultural Science o Kyushu University ma Iapana, he 31 triterpenoids i hoʻokaʻawale ʻia mai ke kino hua o Ganoderma e kāohi i ka neuraminidase o ʻelima mau maʻi influenza A i nā pae like ʻole, aia ʻelua triterpenoids kūpono no ka hoʻomohala ʻana e like me nā lāʻau anti-influenza.Ua paʻi ʻia nā hopena noiʻi ma "Scientific Reports" ma lalo o ka hui hoʻopuka "Nature" ma ka hopena o ʻAukake 2015.

ʻO Neuraminidase kekahi o nā protein ʻelua e puka mai ana ma luna o ka ʻili o nā maʻi maʻi influenza A.Loaʻa i kēlā me kēia maʻi influenza ma kahi o hoʻokahi haneli o kēia mau protease.Ke hoʻokomo ka maʻi maʻi i ka cell a hoʻohana i ka mea i loko o ke kelepona e hana hou i nā mea maʻi virus hou, pono ka neuraminidase no ka wehe ʻana o nā mea maʻi virus hou mai ke kelepona a hoʻopili hou i nā cell ʻē aʻe.No laila, ke nalowale ka neuraminidase i kāna hana, e hoʻopaʻa ʻia ka maʻi hou i loko o ke kelepona a ʻaʻole hiki ke pakele, e hoʻemi ʻia ka hoʻoweliweli i ka mea hoʻokipa, a hiki ke hoʻomalu ʻia ka maʻi.ʻO ka oseltamivir (Tamiflu) i hoʻohana mau ʻia i ka hoʻomaʻamaʻa lapaʻau ʻo ia ka hoʻohana ʻana i kēia kumu no ka pale ʻana i ka laha a me ka hoʻolaha ʻana o ka maʻi.

Wahi a ka noiʻi i alakaʻi ʻia e Kuniyoshi Shimizu, ma kahi ʻano o 200 μM, ua kāohi kēia mau Ganoderma triterpenoids i ka hana o H1N1, H5N1, H7N9 a me ʻelua mau ʻano mutant kūpaʻa NA (H1N1, N295S) a me NA (H3N2, E119V) i nā degere like ʻole.Ma ka holoʻokoʻa, ʻoi aku ka maikaʻi o ka hopena inhibitory ma ka neuraminidase o ka ʻano N1 (ʻoi aku ka H5N1), a ʻo ka hopena hoʻoneʻe i ka neuraminidase o H7N9 ka mea ʻino loa.Ma waena o kēia mau triterpenoids, ʻo ka ganoderic acid TQ a me ka ganoderic acid TR i hōʻike i nā kiʻekiʻe kiʻekiʻe o ka inhibition, a ʻo nā hopena o kēia mau pūhui ʻelua mai ka 55.4% a i ka 96.5% inhibition no nā subtypes NA like ʻole.

ʻO ka nānā hou aku o ka pilina hana-hana o kēia mau triterpenoids ua hōʻike ʻia ʻo nā triterpenoids, i ʻoi aku ka maikaʻi o ka hoʻopā ʻana i ka N1 neuraminidase, loaʻa ke ʻano nui o "tetracyclic triterpenoids me ʻelua mau paʻa pālua, he lālā ma ke ʻano he hui carboxylic, a me kahi oxygen- loaʻa pūʻulu ma ka pūnaewele R5” (Backbone A ma ke kiʻi ma lalo).Inā ʻo ka hale nui nā ʻelua ʻē aʻe (Backbone B a me C ma ke kiʻi ma lalo nei), ʻaʻole maikaʻi ka hopena.

(Source/Sci Rep. 2015 Aug 26;5:13194.)

Hoʻohana ʻia ka docking silico e hoʻohālikelike i ka pilina o nā waikawa ganoderic (TQ a me TR) a me nā neuraminidases (H1N1 a me H5N1).ʻO ka hopena, ua ʻike ʻia ua hiki i nā waikawa ganoderic a me Tamiflu ke hoʻopaʻa pololei i ka wahi hana o neuraminidase.Aia kēia wahi hana i nā koena amino acid.ʻO Ganoderma acids TQ a me TR e hoʻopaʻa i nā koena amino acid ʻelua Arg292 a me Glu119.He koho ʻē aʻe ʻo Tamiflu akā hiki ke hana i ka neuraminidase i mea ʻole.

Ke hoʻohālikelike ʻia me ke kāohi ʻana i nā protein ʻē aʻe i ka maʻi maʻi influenza (e like me ka M2 protein, ka mea e wehe ai i ka pūpū virus i ka manawa e hoʻopaʻa ai ka maʻi i ka cell host a hoʻouna i nā genes viral i ka cell), ʻike ʻia nā neuraminidase inhibitors i kēia manawa he maikaʻi a emi ʻole. lāʻau lapaʻau kūʻē i ka maʻi maʻi.No laila, manaʻo ka poʻe noiʻi e loaʻa ana i ka ganoderic acid TQ a me TR, nā mea like ʻole akā ʻaʻole like i ka mīkini o Tamiflu, e hoʻohana ʻia ma ke ʻano he hanauna hou o nā lāʻau anti-influenza a i ʻole nā ​​kuhikuhi hoʻolālā.

Eia nō naʻe, aia kahi mea e pono ai ka lāʻau lapaʻau e hoʻohana ʻia ma ke ʻano he lāʻau anti-influenza, ʻo ia hoʻi, pono ka lāʻau lapaʻau e pale pono i ka hana hou ʻana o ka maʻi me ka ʻole o ka hoʻopōʻino ʻana i nā cell i loaʻa i ka maʻi.Eia nō naʻe, i nā hoʻokolohua ma nā cell i loaʻa i nā maʻi maʻi ola a me nā laina cell cancer maʻi umauma (MCF-7), ua ʻike ʻia i ka wā i hoʻohana ai nā mea noiʻi i kēia mau ʻano ʻelua o ka ganoderic acid wale nō, ua kānalua lākou no ka cytotoxicity kiʻekiʻe, akā ua loaʻa pū kekahi ʻano ʻē aʻe. o Ganoderma triterpenoid, ganoderol B, loaʻa ka hopena paʻa i ka H5N1 (akā, ʻilihune ka hopena inhibitory), akā ʻaʻole ia he cytotoxic.No laila, manaʻo ka poʻe noiʻi pehea e hoʻomaikaʻi ai i ka palekana o nā waikawa ganoderic TQ a me TR ma o ka hoʻololi ʻana i ke ʻano kemika ʻoiai e mālama mau ana i kā lākou hana ʻana i ka hana neuraminidase.

[Ke kumu] Zhu Q, et al.ʻO ka pale ʻana i ka neuraminidase e Ganoderma triterpenoids a me nā hopena no ka hoʻolālā neuraminidase inhibitor.Sci Rep. 2015 Aug 26;5:13194.doi: 10.1038/srep13194.

HOPE

No ka mea kākau/ Ms. Wu Tingyao
Ua hōʻike ʻo Wu Tingyao i ka ʻike Ganoderma lima mua mai 1999. ʻO ia ka mea kākau oHoola me Ganoderma(i paʻi ʻia ma The People's Medical Publishing House ma ʻApelila 2017).
 
★ Ua paʻi ʻia kēia ʻatikala ma lalo o ka mana kūʻokoʻa o ka mea kākau.★ ʻAʻole hiki ke paʻi hou ʻia nā hana i luna, ʻokiʻoki a hoʻohana ʻia ma nā ʻano ʻē aʻe me ka ʻole o ka ʻae ʻia e ka mea kākau.★ No ka uhaki ʻana i ka ʻōlelo ma luna, e ʻimi ka mea kākau i nā kuleana pili kānāwai.★ Ua kākau ʻia ka kikokikona kumu o kēia ʻatikala ma ka ʻōlelo Pākē e Wu Tingyao a unuhi ʻia i ka ʻōlelo Pelekania e Alfred Liu.Inā loaʻa kekahi ʻokoʻa ma waena o ka unuhi (English) a me ka unuhi mua (Pake), e lanakila ka Pākē mua.Inā he nīnau ka poʻe heluhelu, e ʻoluʻolu e kelepona i ka mea kākau kumu, ʻo Ms. Wu Tingyao.