August 26, 2015 / Malipoti a Sayansi ya Kyushu / Sayansi

Mawu/Wu Tingyao

Gulu lofufuza la Kuniyoshi Shimizu, pulofesa wothandizira ku Institute of Agricultural Sciences ku yunivesite ya Kyushu ku Japan, linatsimikizira kuti 31 triterpenoids olekanitsidwa ndi thupi la fruiting la Ganoderma amalepheretsa neuraminidase ya mavairasi asanu a fuluwenza A ku madigiri osiyanasiyana, pakati pawo pali awiri. triterpenoids ngakhale oyenera chitukuko monga mankhwala odana ndi fuluwenza.Zotsatira zafukufuku zidasindikizidwa mu "Scientific Reports" pansi pa gulu lofalitsa la "Nature" kumapeto kwa August 2015.

Neuraminidase ndi imodzi mwa mapuloteni awiri omwe amatuluka pamwamba pa mavairasi a chimfine A.Kachilombo kakang'ono ka chimfine kali ndi pafupifupi zana limodzi mwa ma proteases.Kachilomboka kakalowa m'selo ndikugwiritsa ntchito zomwe zili muselolo kutengera tinthu tating'ono ta tizilombo tating'onoting'ono, neuraminidase imafunika kuti tinthu tating'onoting'ono ta kachilomboka tichoke m'seloyo ndikuwonjezeranso ma cell ena.Choncho, pamene neuraminidase itaya ntchito yake, kachilombo katsopano kamakhala kotsekedwa mu selo ndipo sikungathe kuthawa, chiwopsezo cha wolandirayo chidzachepetsedwa, ndipo matendawa amatha kulamuliridwa.Oseltamivir (Tamiflu) omwe amagwiritsidwa ntchito kwambiri pochita zachipatala ndikugwiritsa ntchito mfundoyi pofuna kupewa kufalikira ndi kufalikira kwa kachilomboka.

Malinga ndi kafukufuku wopangidwa ndi Kuniyoshi Shimizu, pa ndende ya 200 μM, ma Ganoderma triterpenoids awa amalepheretsa ntchito ya H1N1, H5N1, H7N9 ndi mitundu iwiri yosasinthika ya NA (H1N1, N295S) ndi NA (H3N2, E119V) ku madigiri osiyanasiyana.Pazonse, zolepheretsa za neuraminidase za mtundu wa N1 (makamaka H5N1) ndizo zabwino kwambiri, ndipo zoletsa za neuraminidase za H7N9 ndizoyipa kwambiri.Pakati pa triterpenoids, ganoderic acid TQ ndi ganoderic acid TR adawonetsa milingo yayikulu kwambiri yoletsa, ndipo zotsatira zamagulu awiriwa zidachokera ku 55.4% mpaka 96.5% zoletsa za NA subtypes.

Kuwunikanso kuyanjana kwa magwiridwe antchito a triterpenoids awa kunawonetsa kuti ma triterpenoids, omwe amalepheretsa bwino ku N1 neuraminidase, ali ndi kapangidwe kake ka "tetracyclic triterpenoids yokhala ndi zomangira ziwiri ziwiri, nthambi ngati gulu la carboxylic, ndi oxygen- okhala ndi gulu pamalo a R5” (Msana A pachithunzi pansipa).Ngati dongosolo lalikulu ndi zina ziwiri (Backbone B ndi C mu chithunzi pansipa), zotsatira zake zidzakhala zoipa.

(Source/Sci Rep. 2015 Aug 26; 5:13194.)

Mu silico docking amagwiritsidwa ntchito kuyerekezera kuyanjana kwa ganoderic acid (TQ ndi TR) ndi neuraminidases (H1N1 ndi H5N1).Zotsatira zake, zidapezeka kuti onse ganoderic acid ndi Tamiflu amatha kumangirira mwachindunji kudera logwira ntchito la neuraminidase.Malo ogwira ntchitowa amapangidwa ndi zotsalira zingapo za amino acid.Ma Ganoderma acids TQ ndi TR adzamanga ku zotsalira ziwiri za amino acid Arg292 ndi Glu119.Tamiflu ali ndi njira ina koma angapangitsenso kuti neuraminidase isagwire ntchito.

Poyerekeza ndi kuletsa mapuloteni ena pa fuluwenza virus (monga M2 puloteni, amene amatsegula kachilombo chipolopolo pa nthawi kachilombo kamene kamamangiriza ku gulu selo ndi kutumiza ma jini tizilombo selo), neuraminidase inhibitors panopa anazindikira kuti zothandiza ndi zochepa. mankhwala osamva chimfine.Choncho, ofufuza amakhulupirira kuti ganoderic zidulo TQ ndi TR, amene ali ofanana koma osati chimodzimodzi mu limagwirira Tamiflu, ndi mwayi ntchito mbadwo watsopano wa mankhwala odana ndi fuluwenza kapena maumboni kapangidwe.

Komabe, pali chofunikira kuti mankhwalawa agwiritsidwe ntchito ngati mankhwala odana ndi fuluwenza, ndiye kuti, mankhwalawa ayenera kuletsa kubereka kwa kachilomboka popanda kuvulaza maselo omwe ali ndi kachilomboka.Komabe, poyesera ma cell omwe ali ndi ma virus amoyo ndi ma cell a khansa ya m'mawere (MCF-7), zidapezeka kuti ofufuza atagwiritsa ntchito mitundu iwiriyi ya ganoderic acid yokha, anali ndi kukaikira za cytotoxicity yayikulu, koma adapezanso mtundu wina. wa Ganoderma triterpenoid, ganoderol B, ali ndi inhibitory zotsatira pa H5N1 (koma inhibitory zotsatira zake ndi osauka), koma si cytotoxic.Choncho, ochita kafukufuku amakhulupirira kuti momwe angasinthire chitetezo cha ganoderic acids TQ ndi TR kupyolera mu kusintha kwa mankhwala pamene akusungabe zoletsa zawo za neuraminidase ziyenera kuganiziridwa mosamala.

[Source] Zhu Q, et al.Kuletsa kwa neuraminidase ndi Ganoderma triterpenoids ndi zotsatira za neuraminidase inhibitor design.Sci Rep. 2015 Aug 26; 5:13194.doi: 10.1038/srep13194.

TSIRIZA

Za wolemba/ Mayi Wu Tingyao
Wu Tingyao wakhala akufotokoza zambiri za Ganoderma kuyambira 1999. Iye ndi mlembi wa bukuli.Kuchiza ndi Ganoderma(lofalitsidwa mu The People's Medical Publishing House mu April 2017).
 
★ Nkhaniyi yasindikizidwa pansi pa chilolezo cha wolemba.★ Ntchito zomwe zili pamwambazi sizingathe kupangidwanso, kuchotsedwa kapena kugwiritsidwa ntchito mwanjira ina popanda chilolezo cha wolemba.★ Pakuphwanya mawu omwe ali pamwambawa, wolemba adzatsata maudindo oyenera azamalamulo.★ Zolemba zoyambirira za nkhaniyi zidalembedwa m'Chitchaina ndi Wu Tingyao ndikumasulira m'Chingerezi ndi Alfred Liu.Ngati pali kusiyana kulikonse pakati pa kumasulira (Chingerezi) ndi choyambirira (Chitchaina), Chitchaina choyambirira chidzapambana.Ngati owerenga ali ndi mafunso, lemberani wolemba woyamba, Mayi Wu Tingyao.