Ọgọst 26, 2015 / Mahadum Kyushu / Akụkọ sayensị

Ederede/Wu Tingyao

Otu nyocha nke Kuniyoshi Shimizu, onye osote prọfesọ na Institute of Agricultural Sciences nke Mahadum Kyushu dị na Japan, kwadoro na triterpenoids 31 dịpụrụ adịpụ site na mkpụrụ osisi Ganoderma na-egbochi neuraminidase nke nje influenza A n'ụzọ dịgasị iche iche, n'ime ha nwere abụọ. triterpenoids ọbụna dabara maka mmepe dị ka ọgwụ mgbochi influenza.E bipụtara nsonaazụ nyocha na " Akụkọ sayensị" n'okpuru otu mbipụta "Nature" na njedebe nke August 2015.

Neuraminidase bụ otu n'ime protein abụọ na-apụta n'elu nje influenza A.Nje virus ọ bụla influenza nwere ihe dị ka otu narị n'ime protease ndị a.Mgbe nje virus na-abanye n'ime cell ma jiri ihe dị n'ime cell megharịa mkpụrụ ndụ nje ọhụrụ, a na-achọ neuraminidase maka nje virus ọhụrụ ahụ ka ọ pụọ na cell ma na-ebutewanye mkpụrụ ndụ ndị ọzọ.Ya mere, mgbe neuraminidase kwụsịrị ọrụ ya, a ga-akpọchi nje virus ọhụrụ ahụ na cell ma ghara ịgbanarị, egwu egwu nke onye ọbịa ahụ ga-ebelata, a pụkwara ịchịkwa ọrịa ahụ.Oseltamivir (Tamiflu) a na-ejikarị eme ihe na ụlọ ọgwụ bụ iji ụkpụrụ a gbochie mgbasa na mgbasa nke nje.

Dika nyocha nke Kuniyoshi Shimizu mere, na ntinye nke 200 μM, ndị Ganoderma triterpenoids gbochiri ọrụ H1N1, H5N1, H7N9 na ụdị mutant abụọ na-eguzogide NA (H1N1, N295S) na NA (H3N2, E119V) na ogo dịgasị iche.N'ozuzu ya, mmetụta mgbochi na neuraminidase nke ụdị N1 (karịsịa H5N1) bụ ihe kachasị mma, na mmetụta mgbochi na neuraminidase nke H7N9 bụ nke kachasị njọ.N'ime triterpenoids ndị a, ganoderic acid TQ na ganoderic acid TR gosipụtara ọkwa kachasị elu nke mgbochi, na mmetụta nke ogige abụọ a sitere na 55.4% ruo 96.5% mgbochi maka ụdị NA dị iche iche.

Nyocha ọzọ nke nhazi-mmekọrịta mmekọrịta nke triterpenoids ndị a kpughere na triterpenoids, nke nwere mmetụta mgbochi ka mma na N1 neuraminidase, nwere isi nhazi nke "tetracyclic triterpenoids nwere njikọ abụọ, alaka dị ka otu carboxylic, na oxygen- nwere otu na saịtị R5” (Ọkpụkpụ azụ A na foto dị n'okpuru).Ọ bụrụ na usoro bụ isi bụ abụọ ndị ọzọ (ọkpụkpụ B na C na ọnụ ọgụgụ dị n'okpuru ebe a), mmetụta ahụ ga-adị njọ.

(Isi mmalite/Sci Rep. 2015 Ọgọst 26;5:13194.)

A na-eji docking silico mee ka mmekọrịta nke ganoderic acid (TQ na TR) na neuraminidases (H1N1 na H5N1).N'ihi ya, a chọpụtara na ma ganoderic acid na Tamiflu nwere ike ijikọ kpọmkwem na mpaghara neuraminidase na-arụ ọrụ.Mpaghara a na-arụ ọrụ nwere ọtụtụ ihe fọdụrụ na amino acid.Ganoderma acid TQ na TR ga-ejikọta na amino acid abụọ Arg292 na Glu119.Tamiflu nwere nhọrọ ọzọ mana ọ nwekwara ike ime ka neuraminidase ghara ịdị irè.

E jiri ya tụnyere igbochi protein ndị ọzọ na nje influenza (dị ka protein M2, nke na-emepe shea nje n'oge nje virus na-ejikọta ya na cell cell ma na-eziga mkpụrụ ndụ ihe nketa na cell), ndị na-egbochi neuraminidase bụ ndị a na-amata ugbu a dị ka ndị dị irè ma dị obere. ọgwụ ọgwụgwọ influenza na-eguzogide ọgwụ.Ya mere, ndị nchọpụta kwenyere na ganoderic acids TQ na TR, bụ ndị yiri ma ọ bụghị otu n'ime usoro Tamiflu, nwere ohere iji mee ihe dị ka ọgbọ ọhụrụ nke ọgwụ mgbochi influenza ma ọ bụ nkọwa nhazi.

Otú ọ dị, e nwere ihe a chọrọ iji mee ọgwụ ahụ dị ka ọgwụ mgbochi influenza, ya bụ, ọgwụ ahụ aghaghị igbochi mmeputakwa nke nje n'ụzọ dị irè na-emerụghị sel ndị nje ahụ butere.Otú ọ dị, na nyocha nke mkpụrụ ndụ ndị nwere nje ndị dị ndụ na ọrịa cancer ara (MCF-7), a chọpụtara na mgbe ndị nchọpụta ahụ jiri ụdị ganoderic acid abụọ a naanị, ha nwere obi abụọ banyere nnukwu cytotoxicity, ma ha chọtakwara ụdị ọzọ. nke Ganoderma triterpenoid, ganoderol B, nwere mmetụta mgbochi na H5N1 (mana mmetụta mgbochi adịghị mma), ma ọ bụghị cytotoxic.Ya mere, ndị nchọpụta kwenyere na otu esi emeziwanye nchekwa nke ganoderic acids TQ na TR site na mgbanwe nke usoro kemịkalụ ka ha na-ejigide mgbochi ha nke ọrụ neuraminidase ga-echebara nke ọma echiche.

[Isi mmalite] Zhu Q, et al.Mgbochi nke neuraminidase site na Ganoderma triterpenoids na ihe ọ pụtara maka imepụta ihe mgbochi neuraminidase.Sci Rep. 2015 Ọgọst 26;5:13194 .doi: 10.1038/srep13194.

N'ikpeazụ

Banyere onye odee/ Oriakụ Wu Tingyao
Wu Tingyao na-akọ akụkọ gbasara Ganoderma nke mbụ kemgbe 1999. Ọ bụ ya bụ onye dere akwụkwọ akụkọ.Ọgwụgwọ na Ganoderma(nke e bipụtara na The People's Medical Publishing House na Eprel 2017).
 
★ A na-ebipụta akụkọ a n'okpuru ikike nke onye edemede.★ Enweghị ike imepụtagharị ọrụ ndị a dị n'elu, wepụ ma ọ bụ jiri ya mee ihe n'ụzọ ndị ọzọ na-enweghị ikike nke onye edemede.★ Maka mmebi nke nkwupụta a dị n'elu, onye edemede ga-agbaso ọrụ iwu dị mkpa.★ Wu Tingyao dere ederede izizi nke akụkọ a n'asụsụ Chinese wee sụgharịa ya n'asụsụ Bekee site n'aka Alfred Liu.Ọ bụrụ na enwere nghọtahie n'etiti ntụgharị asụsụ (Bekee) na nke izizi (Chinese), Chinese izizi ga-emeri.Ọ bụrụ na ndị na-agụ akwụkwọ nwere ajụjụ ọ bụla, biko kpọtụrụ onye edemede mbụ bụ Oriakụ Wu Tingyao.