Ngomhla we-8 kaNovemba, 2020/iKholeji yezoNyango, iYunivesithi yaseTibet/iBiology yezamayeza

Umbhalo/Wu Tingyao

Ngaba izigulane ezinomhlaza zingathathaI-Ganoderma lucidumngelixa ufumana unyango olujoliswe kuyo?Ndiyathemba ukuba le ngxelo yophando ilandelayo inokunika iimpendulo.

I-Gefitinib (GEF) lelinye lawona machiza abalulekileyo ekujoliswe kuwo kunyango lomhlaza wemiphunga okwinqanaba eliphezulu kunye nemetastatic engeyiyo encinci (kubandakanya i-lung adenocarcinoma, umhlaza we-squamous cell lung, kunye nomhlaza wemiphunga omkhulu weseli), ezisa ithemba kwizigulane ezithe zafumana ithemba. basinda ebumnyameni.Kodwa ukukhanya ekuphumeni kwetonela akunakusoloko kukhanya, kuba ukunganyangeki ngamachiza kuthanda ukuvela emva kweenyanga ezilishumi ukuya kwezilishumi elinesithandathu zonyango.

Ngoko ke, ukuba sinokulibamba ixesha lokuphucula isiphumo sonyango lwe-GEF, zama ukunyanga umhlaza wemiphunga ukuya kwimeko elawulekayo kunye nokugcinwa ngcono okanye ukunciphisa iziphumo ebezingalindelekanga zeziyobisi ukuze izigulane zibe nemeko engcono yomzimba ukujongana nayo. umhlaza, mhlawumbi kukho ithuba lokwenza ukukhanya kobomi kukhanye ngakumbi.

Abaphandi abavela kwiSebe le-Oncology yeSibhedlele saseYantai seNyanga yesiNtu yaseTshayina kunye neKholeji yezoNyango yeYunivesithi yaseTibet ngokudibeneyo bapapashe ingxelo yophando kwi "Pharmaceutical Biology" ekupheleni kuka-2020 ebonakalise ngovavanyo lwezilwanyana ukuba yeyona adenocarcinoma yemiphunga ixhaphakileyo kwindawo engencinci. umhlaza wemiphunga yeseli, ukusetyenziswa okudityanisiweyoI-Ganodermalucidumi-triterpenoids (GLTs) kunye ne-GEF inokuthintela ngokufanelekileyo ukukhula kwethumba kunye nokunciphisa iziphumo ebezingalindelekanga zamachiza, zibonelela ngesicwangciso esitsha esifanele ukuqwalaselwa kwizicwangciso zonyango ezinxulumeneyo.

Abaphandi baqala ukufaka imigca yeseli ye-alveolar adenocarcinoma (i-A549 cell lines) phantsi kwesikhumba seempuku kunye ne-immune system.Emva kokuba i-diameters ye-subcutaneous tumors yayimalunga ne-6-8 mm, baqala ukutyaI-Ganoderma lucidumi-triterpenoids (i-GLT, i-1 g / kg / ngosuku), i-gefitinib (GEF, i-15 mg / kg / ngosuku) okanye ukudibanisa zombini iintsuku ze-14, kwaye uvavanyo lwaphela ngomhla we-15.Kwavela ukuba:

(1) Ukuphucula ukukhula kwe-tumor rate inhibition rate

I-GLTs kunye ne-GEF inokuthintela ukukhula kwe-lung adenocarcinoma tumors, kodwa ukudibanisa kwezi zibini kunempembelelo engcono (Umfanekiso 1 ~ 3).

Umzobo 1 Amathumba athatyathwe kwiimpuku ze-lung adenocarcinoma ekupheleni kovavanyo

Umzobo we-2 Utshintsho ekukhuleni kwethumba leempuku ze-lung adenocarcinoma ngexesha lovavanyo

Umzobo 3 Izinga lokuthintela ukukhula kwethumba kwiimpuku ze-lung adenocarcinoma ngeendlela ezahlukeneyo zonyango.

2) Ukuqinisa ukuvinjelwa kwe-tumor angiogenesis kunye nokukhuthazwa kwe-apoptosis yeseli yomhlaza

Amathumba kufuneka enze iinqanawa ezintsha ukuze ziqhubeke zikhula.Ke ngoko, ukuxinana kwee-microvessels kwizicubu ze-tumor kuye kwaba sisitshixo esibalulekileyo ekukhuleni okugudileyo kwamathumba.Umfanekiso we-4 (A) ubonisa ukuhanjiswa kwee-microvessels kwizilayi zezicubu zethumba kwiqela ngalinye.Umzobo we-4 (B) ubonisa ukuba ukudibanisa kwe-GLTs kunye ne-GEF kunempembelelo engcono yokuthintela kunezo zimbini zodwa.

Umzobo 4 Amacandelo ezicubu zethumba kunye nokuxinana kwe-microvessel yeempuku ze-lung adenocarcinoma

Ngamanye amazwi, indibaniselwano ye-GLTs kunye ne-GEF inokuthintela izicubu ezininzi ze-tumor ekufumaneni izondlo kwaye zenze amathumba abe nzima ukukhula.Le ndlela yokusebenza ivela kummiselo oqinisiweyo we-gene expression kunye ne-protein secretion kwi-tumor tissues, kubandakanywa nokuvimbela "i-vascular endothelial growth factor receptor 2 (VEGFR2)" kunye nokukhuthaza ukuveliswa kwe "Angiostatin" kunye ne "endostatin".

Ukongeza, abaphandi baye baqaphela kumacandelo ezicubu zethumba kwiqela ngalinye leempuku ukuba phantsi kwesenzo esidibeneyo se-GLTs kunye ne-GEF, i-secretion yeprotheyini (i-Bax) ekhuthaza i-apoptosis yeseli yomhlaza iya kwanda kakhulu ngelixa i-secretion yeprotheni (Bcl- 2) inqanda i-apoptosis yeeseli zomhlaza ziya kuncipha.Iiseli ze-lung adenocarcinoma ziyakhawuleza ekuphuhliseni ukuya kwicala le-apoptosis kule plus kunye ne-minus force.

(3) Ukunciphisa imiphumo emibi yeziyobisi

Imiphunga ye-adenocarcinoma ye-Lung eyanyangwa kuphela nge-GEF yaba nelahleko enkulu yobunzima;ngakolunye uhlangothi, ukudibanisa kwe-GLTs kunye ne-GEF kunokugcina kakuhle ubunzima bomzimba weegundane ze-lung adenocarcinoma ── kufutshane naleyo yeegundane eziqhelekileyo (iqela eliqhelekileyo lokulawula) (Umfanekiso 5).

Ukongeza, iimpuku ze-lung adenocarcinoma eziphathwa kuphela nge-GEF zibonise ukuxhalaba, ukudinwa, ukozela, ukuncipha komsebenzi, ukunciphisa ukutya kunye nolusu oluthambileyo.Nangona kunjalo, ezi meko bezikhaphukhaphu kakhulu okanye zingabonakali kwiqela eliphathwe ngokudityaniswa kwe-GLTs kunye ne-GEF.Ngokucacileyo, ii-GLTs zinokulungisa iziphumo ebezingalindelekanga ezibangwa yi-GEF.

Umzobo we-5 Iigophe zeerekhodi zobunzima kunye notshintsho kwiigundane ze-lung adenocarcinoma ngexesha lovavanyo

(4) Ukhuseleko lwe-GLTs

Ukuze kuhlolwe ukhuseleko lwe-GLTs, abaphandi bahlakulela imigca yeseli ye-alveolar epithelial eqhelekileyo ye-BEAS-2B kunye nemigca yeseli ye-alveolar adenocarcinoma ye-A549 esetyenziswa kwiimvavanyo zezilwanyana ngokulandelelana kunye ne-GLTs in vitro iiyure ze-48.

Iziphumo ziboniswe kuMzobo 6. Xa i-GLTs (i-concentrations ye-2.5 kunye ne-5 mg / L) ivimbele izinga lokusinda kweeseli ze-lung adenocarcinoma ukuya kwi-80-60%, iiseli eziqhelekileyo zazisaphila;nakwiindawo eziphezulu, ii-GLT zisaphatha ngokucacileyo iiseli zomhlaza kunye neeseli eziqhelekileyo ngokwahlukileyo, kwaye lo mahluko ubaluleke ngakumbi kune-GEF (Umfanekiso 7).

Umzobo we-6 Umphumela wokuthintela we-GLTs ekukhuleni kweeseli

Umzobo 7 Impembelelo yokuthintela i-gefitinib ekukhuleni kweeseli

Ngokohlalutyo lomphandi, ixabiso le-IC50 ye-GLTs kwi-48 h yonyango lwe-A549 imigca yeseli yayiyi-14.38 ± 0.29 mg / L ngelixa i-GLTs ibonise umphumo ongaphantsi kakhulu we-cytotoxic kwi-BEAS-2B umgca weseli kunye nexabiso le-IC50 le-78.62 ± 2.53 mg / L, oku kuthetha ukuba xa ii-GLTs zibulala iiseli zomhlaza, zisenokugcina izinga eliphezulu lokhuseleko kwiiseli eziqhelekileyo.

I-GLTs kunye nonyango olujoliswe kuyo luhamba kunye, okwenza unyango luthembise ngakumbi.

Le ngxelo yophando isibonisile:

Phantsi kweemeko zovavanyo ezifanayo, ulawulo lomlomo lwe-GLTs lusenokungabi nempembelelo efanayo yokuthintela kwimiphunga ye-adenocarcinoma yemiphunga yabantu njenge-GEF, kodwa ii-GLTs azinaziphumo bezingalindelekanga ze-GEF.

Xa i-GLTs kunye ne-GEF zisebenza kunye, azikwazi ukunyusa kuphela umphumo wokuthintela ekukhuleni kwe-tumor kodwa zinciphisa imiphumo ye-gefitinib kubunzima, umoya, amandla, ukutya kunye nolusu.Oku kubizwa ngokuba "ukwandisa ukusebenza kakuhle kunye nokunciphisa ubuthi".

Isizathu sokuba kutheni i-GLTs inokuphucula inhibition ye-GEF ye-lung adenocarcinoma tumors inxulumene "nokuthintela i-tumor angiogenesis" kunye "nokukhuthaza i-apoptosis yeseli yomhlaza".

Ukuze kuvavanywe umhlaza womntu kwizilwanyana, abaphandi basebenzisa iigundane ezinezistim zokuzikhusela (ukuze iiseli zomhlaza womntu zikhule kwiintlobo ezahlukeneyo).Ke ngoko, iziphumo beziyimpembelelo ye-GLTs kunye ne-GEF ngokwayo kwiiseli zomhlaza.

Nangona kunjalo, ekusetyenzisweni kwangempela kwe-anti-cancer, umsebenzi we-immune system kufuneka ubandakanyeke.Ngoko ke, ngaphezu kwe-GLTs kunye ne-GEF, ukuba "i-immunity enhle" yongezwa, ngaba iziphumo ziya kuba zikhangele ngakumbi?

Abaphandi abazange banike inkcazo eninzi ye-GLTs esetyenziswe kuvavanyo, kodwa ngokwenkcazo yephepha, kufuneka ibe yi-extract crude ye-GLTs ezahlukeneyo.Kodwa idosi esebenzayo yegram enye ngekhilogram yobunzima bomzimba kwiimpuku ininzi kakhulu.Oku kusixelela ukuba usetyenziso olusebenzayo lusenokufuna ithamo elikhulu ukuze lisebenze.Ngakolunye uhlangothi, kusinika ithemba lokuba kwixesha elizayo kunokwenzeka ukufumana izithako eziphambili ezinokusebenza kakuhle okanye ezingcono kwiidosi ezisezantsi.

Kuyo nayiphi na imeko, ubuncinci olu phando lubonise ukuba i-triterpenoids evela kwi-Ganoderma lucidum ayithinteli nje kuphela unyango lwamachiza asetyenziswa ngokuqhelekileyo ekliniki kodwa ibe nefuthe elihle "lokwandisa ukusebenza kakuhle kunye nokunciphisa ubuthi" ngokusekelwe kukhuseleko oluluqilima.
Ukugrogrisa kwitonela elimnyama kufuna ukukhanya kwekhandlela ngakumbi ukukhokela indlela kunye nokukhanyisa.Xa kuthelekiswa nalawo "themba" angenakufikeleleka okanye kunzima ukuvelisa ngobuninzi, okanye "iindlela zokupheka eziyimfihlo" ezinemithombo engaziwayo kunye nezithako,I-Ganoderma lucidumi-triterpenoids, enokuthi ifumaneke ixesha elide njengoko ufuna kwaye uqokelele amava okusebenzisa ixesha elide, kufuneka ifanele ukuzama.

[Umthombo] Wei Liu, et al.I-Ganoderma triterpenoids ithomalalisa i-tumor angiogenesis kumhlaza wemiphunga ezithwele ithumba iimpuku ezinqunu.Pharm Biol.2020: 58(1): 1061-1068.

ISIPHELO

Malunga nombhali/ Nksz. Wu Tingyao
UWu Tingyao unike ingxelo ngolwazi lweGanoderma lokuqala ukusukela ngo-1999. Ungumbhali wencwadiUkunyanga ngeGanoderma(ipapashwe kwiNdlu yePeople's Medical Publishing House ngo-Epreli 2017).
 
★ Eli nqaku lipapashwe phantsi kogunyaziso olulodwa lombhali.★ Le misebenzi ingasentla ayinakuphinda iveliswe, icatshulwe okanye isetyenziswe ngezinye iindlela ngaphandle kokugunyaziswa ngumbhali.★ Ukwaphulwa kwengxelo engentla, umbhali uya kulandela uxanduva olusemthethweni olufanelekileyo.★ Umbhalo wokuqala weli nqaku ubhalwe ngesiTshayina nguWu Tingyao waza waguqulelwa kwisiNgesi nguAlfred Liu.Ukuba kukho nakuphi na ukungangqinelani phakathi kwenguqulelo (isiNgesi) kunye neyoqobo (isiTshayina), isiTshayina sokuqala siya koyisa.Ukuba abafundi banayo nayiphi na imibuzo, nceda uqhagamshelane nombhali wokuqala, uNks. Wu Tingyao.