November 8, 2020/Medical College, Tibet University/Pharmaceutical Biology

Mawu/Wu Tingyao

Kodi odwala khansa angatengeGanoderma lucidumpamene akulandira chithandizo chandamale?Tikukhulupirira kuti lipoti lotsatirali lingapereke mayankho.

Gefitinib (GEF) ndi amodzi mwamankhwala ofunikira kwambiri pochiza khansa yapakhungu yomwe siing'ono kwambiri (kuphatikiza khansa ya m'mapapo ya m'mapapo, khansa ya m'mapapo ya squamous cell, ndi khansa yayikulu ya m'mapapo), zomwe zimabweretsa chiyembekezo kwa odwala omwe ali ndi matenda ashuga. akupulumuka mumdima.Koma kuwala kotuluka mumphangayo sikungakhale koyaka nthawi zonse, chifukwa kusamva mankhwala kumayamba pambuyo pa miyezi khumi mpaka khumi ndi isanu ndi umodzi ya chithandizo.

Chifukwa chake, ngati titha kugwiritsa ntchito nthawi kuti tithandizire kuchiritsa kwa GEF, yesetsani kuchiza khansa ya m'mapapo kuti ikhale yokhazikika komanso yosamaliridwa bwino kapena kuchepetsa zotsatirapo za mankhwala kuti odwala athe kukhala ndi thanzi labwino kuti athane nawo. khansara, mwinamwake pali mwayi wopangitsa kuwala kwa moyo kuwalitsa kwambiri.

Ofufuza ochokera ku dipatimenti ya Oncology ku Yantai Hospital of Traditional Chinese Medicine ndi Medical College ya Tibet University pamodzi adafalitsa lipoti la kafukufuku mu "Pharmaceutical Biology" kumapeto kwa 2020 zomwe zidatsimikizira kudzera mu kuyesa kwa nyama kuti kwa ambiri m'mapapo adenocarcinoma omwe si ang'onoang'ono. khansa ya m'mapapo, kugwiritsa ntchito pamodziGanodermalucidumtriterpenoids (GLTs) ndi GEF amatha kulepheretsa kukula kwa chotupa ndi kuchepetsa zotsatira za mankhwala, kupereka dongosolo latsopano loyenera kulingalira za njira zothandizira zothandizira.

Ofufuzawo adayikapo ma cell a alveolar adenocarcinoma cell (mizere ya A549 cell) pansi pa khungu la mbewa zokhala ndi chitetezo chofooka.Pambuyo pa ma diameter a subcutaneous zotupa pafupifupi 6-8 mm, adayamba kudyetsaGanoderma lucidumtriterpenoids (GLT, 1 g / kg / tsiku), gefitinib (GEF, 15 mg / kg / tsiku) kapena kuphatikiza kwa masiku 14, ndipo kuyesa kunatha pa tsiku la 15.Zinapezeka kuti:

(1) Sinthani kuchuluka kwa chotupa choletsa kukula

GLTs ndi GEF zingalepheretse kukula kwa zotupa za m'mapapo adenocarcinoma, koma kuphatikiza kwa ziwirizi kumakhala ndi zotsatira zabwino (Chithunzi 1 ~ 3).

Chithunzi 1 Zotupa zotengedwa m'mapapo adenocarcinoma mbewa kumapeto kwa kuyesa

Chithunzi 2 Kusintha kwa kukula kwa chotupa cha mbewa zamapapu adenocarcinoma panthawi yoyesera

Chithunzi 3 Chotupa choletsa kukula kwa mbewa zamapapu adenocarcinoma ndi njira zosiyanasiyana zochizira

2) Limbikitsani chopinga cha chotupa angiogenesis ndi kulimbikitsa khansa cell apoptosis

Zotupa ziyenera kupanga zombo zatsopano kuti zipitirire kukula.Chifukwa chake, kuchuluka kwa ma microvessels muzotupa za chotupa kwakhala kiyi yofunikira pakukula kosalala kwa zotupa.Chithunzi 4 (A) chikuwonetsa kugawidwa kwa ma microvessels mu magawo a chotupa cha gulu lililonse.Chithunzi 4 (B) chikuwonetsa kuti kuphatikiza kwa GLTs ndi GEF kumakhala ndi zotsatira zabwino zoletsa kuposa awiri okhawo.

Chithunzi 4 Zigawo za minofu ya chotupa ndi kachulukidwe kakang'ono ka mapapu adenocarcinoma mbewa

Mwanjira ina, kuphatikiza kwa GLTs ndi GEF kumatha kuletsa minofu yambiri yotupa kuti isapeze zakudya komanso kupangitsa kuti zotupa zikhale zovuta kukula.Kachitidwe kameneka kameneka kamachokera ku kukhazikitsidwa kwa kulimbikitsidwa kwa ma jini okhudzana ndi kutulutsa mapuloteni m'matumbo a chotupa, kuphatikizapo kuletsa "vascular endothelial growth factor receptor 2 (VEGFR2)" ndikulimbikitsa kupanga "Angiostatin" ndi "endostatin".

Kuphatikiza apo, ofufuzawo adawonanso m'magawo a minofu ya chotupa pagulu lililonse la mbewa kuti molumikizana ndi GLTs ndi GEF, katulutsidwe ka mapuloteni (Bax) omwe amalimbikitsa apoptosis ya khansa kumawonjezeka kwambiri pomwe kutulutsa kwa mapuloteni (Bcl- 2) yomwe imalepheretsa apoptosis ya maselo a khansa idzachepa.Maselo a m'mapapo adenocarcinoma amafulumira kupititsa patsogolo njira ya apoptosis mu mphamvu iyi yowonjezera komanso yochepetsera.

(3) Kuchepetsa zotsatira za mankhwala

Mapapo adenocarcinoma mbewa zomwe zimangochitidwa ndi GEF zinali zowonda kwambiri;Kumbali inayi, kuphatikiza kwa GLTs ndi GEF kumatha kusunga bwino thupi la mbewa zamapapu adenocarcinoma ── pafupi kwambiri ndi mbewa zabwinobwino (gulu lowongolera bwino) (Chithunzi 5).

Komanso, mapapo adenocarcinoma mbewa okha mankhwala ndi GEF anasonyeza nkhawa, kutopa, kugona, utachepa ntchito, kuchepetsa chilakolako cha kudya ndi kuzimiririka khungu.Komabe, mikhalidwe iyi inali yopepuka kwambiri kapena yosadziwika bwino m'gulu lomwe limathandizidwa ndi kuphatikiza kwa GLTs ndi GEF.Mwachiwonekere, GLTs imatha kukonza zotsatira zoyipa zomwe zimayambitsidwa ndi GEF.

Chithunzi 5 Ma Curves of weight records and change in lung adenocarcinoma mbewa panthawi yoyesera

(4) Chitetezo cha GLTs

Pofuna kuwunika chitetezo cha GLTs, ofufuzawo adapanga mizere yodziwika bwino ya alveolar epithelial cell ya BEAS-2B ndi mizere ya alveolar adenocarcinoma cell A549 yomwe imagwiritsidwa ntchito poyesa nyama motsatana ndi GLTs mu vitro kwa maola 48.

Zotsatira zikuwonetsedwa mu Chithunzi 6. Pamene GLTs (zokhazikika za 2.5 ndi 5 mg / L) zinalepheretsa kupulumuka kwa maselo a mapapu adenocarcinoma ku 80-60%, maselo abwinobwino anali amoyo;ngakhale pazigawo zambiri, GLTs amachitirabe bwino maselo a khansa ndi maselo abwinobwino mosiyana, ndipo kusiyana kumeneku ndikofunika kwambiri kuposa GEF (Chithunzi 7).

Chithunzi 6 Mphamvu yoletsa ya GLTs pakukula kwa ma cell

Chithunzi 7 Mphamvu yoletsa ya gefitinib pakukula kwa ma cell

Malinga ndi kusanthula kwa ofufuza, ma IC50 a GLTs pa 48 h ya chithandizo cha ma cell a A549 anali 14.38 ± 0.29 mg/L pomwe GLTs adawonetsa mphamvu ya cytotoxic pa cell ya BEAS-2B yokhala ndi mtengo wa IC50 wa 78.62 ± 2.53 mg / L, zomwe zikutanthauza kuti pamene GLTs ali oopsa ku maselo a khansa, amatha kukhalabe otetezeka kwambiri ku maselo abwinobwino.

GLTs ndi chithandizo chomwe akuchifuna chimayendera limodzi, zomwe zimapangitsa kuti chithandizocho chikhale chodalirika.

Lipoti la kafukufukuyu latiwonetsa:

Pansi pa zoyeserera zomwezo, kuwongolera pakamwa kwa GLTs sikungakhale ndi choletsa chofanana pa zotupa za m'mapapo adenocarcinoma monga GEF, koma GLTs alibe zotsatira zoyipa za GEF.

Pamene GLTs ndi GEF amagwirira ntchito limodzi, sangangowonjezera zotsatira zolepheretsa kukula kwa chotupa komanso kuchepetsa zotsatira za gefitinib pa kulemera, mzimu, mphamvu, chilakolako ndi khungu.Izi ndi zomwe zimatchedwa "kuwonjezera mphamvu ndi kuchepetsa kawopsedwe".

Chifukwa chomwe GLTs angathandizire kuletsa kwa GEF kuletsa zotupa za m'mapapo adenocarcinoma zimagwirizana ndi "kuletsa angiogenesis yotupa" komanso "kulimbikitsa apoptosis ya khansa".

Pofuna kuyesa khansa yaumunthu mu nyama, ofufuzawo adagwiritsa ntchito mbewa zomwe zili ndi chitetezo chamthupi chofooka (kuti maselo a khansa ya anthu akule pamitundu yosiyanasiyana).Chifukwa chake, zotsatira zake zinali zotsatira za GLTs ndi GEF palokha pama cell a khansa.

Komabe, pogwiritsira ntchito kwenikweni anti-cancer, ntchito ya chitetezo cha mthupi iyenera kuphatikizidwa.Choncho, kuwonjezera pa GLTs ndi GEF, ngati "chitetezo chabwino" chikuwonjezeredwa, kodi zotsatira zake zidzakhala zokopa kwambiri?

Ofufuzawo sanafotokoze zambiri za GLTs zomwe zimagwiritsidwa ntchito poyesera, koma malinga ndi kufotokozera kwa pepala, ziyenera kukhala zowonongeka za GLTs zosiyanasiyana.Koma mlingo wogwira mtima wa gramu imodzi pa kilogalamu ya kulemera kwa thupi mu mbewa ndi wochuluka kwambiri.Izi zikutiuza kuti kugwiritsa ntchito moyenera kungafunike mlingo wokwanira kuti ukhale wogwira mtima.Kumbali ina, zimatipatsanso chiyembekezo kuti m'tsogolomu zitha kukhala zotheka kupeza zosakaniza zazikulu zomwe zingagwire ntchito bwino kapena bwino pamilingo yocheperako.

Mulimonsemo, osachepera kafukufukuyu wasonyeza kuti ma triterpenoids ochokera ku Ganoderma lucidum sikuti amangolepheretsa chithandizo chamankhwala omwe amagwiritsidwa ntchito kawirikawiri komanso amakhala ndi zotsatira zabwino za "kuwonjezera mphamvu ndi kuchepetsa kawopsedwe" potengera chitetezo chokwanira.
Kuyenda mumsewu wamdima kumafuna kuwala kwa makandulo kuti kutsogolera njira ndikuwunikira.Poyerekeza ndi "ziyembekezo" zomwe sizikutheka kapena zovuta kupanga zambiri, kapena "maphikidwe achinsinsi" omwe ali ndi magwero osadziwika ndi zosakaniza,Ganoderma lucidumtriterpenoids, yomwe ingapezeke malinga ndi momwe mukufunira ndipo mwakhala ndi nthawi yayitali yogwiritsira ntchito, iyenera kukhala yoyenera kuyesa.

[Source] Wei Liu, et al.Ganoderma triterpenoids attenuate chotupa angiogenesis mu khansa ya m'mapapo yokhala ndi chotupa mbewa zamaliseche.Zotsatira Pharm Biol.2020: 58(1): 1061-1068.

TSIRIZA

Za wolemba/ Mayi Wu Tingyao
Wu Tingyao wakhala akufotokoza zambiri za Ganoderma kuyambira 1999. Iye ndi mlembi wa bukuli.Kuchiza ndi Ganoderma(lofalitsidwa mu The People's Medical Publishing House mu April 2017).
 
★ Nkhaniyi yasindikizidwa pansi pa chilolezo cha wolemba.★ Ntchito zomwe zili pamwambazi sizingathe kupangidwanso, kuchotsedwa kapena kugwiritsidwa ntchito mwanjira ina popanda chilolezo cha wolemba.★ Pakuphwanya mawu omwe ali pamwambawa, wolemba adzatsata maudindo oyenera azamalamulo.★ Zolemba zoyambirira za nkhaniyi zidalembedwa m'Chitchaina ndi Wu Tingyao ndikumasulira m'Chingerezi ndi Alfred Liu.Ngati pali kusiyana kulikonse pakati pa kumasulira (Chingerezi) ndi choyambirira (Chitchaina), Chitchaina choyambirira chidzapambana.Ngati owerenga ali ndi mafunso, lemberani wolemba woyamba, Mayi Wu Tingyao.