Mbudzi 8, 2020/Medical College, Tibet University/Pharmaceutical Biology

Chinyorwa/Wu Tingyao

Varwere vegomarara vanogona kutoraGanoderma lucidumuchigamuchira kurapwa kwakanangwa?Ndinovimba tsvakiridzo inotevera inogona kupa dzimwe mhinduro.

Gefitinib (GEF) ndeimwe yemishonga inonyanya kukosha yekurapa kwepamusoro uye metastatic isiri diki kenza yemapapu (kusanganisira lung adenocarcinoma, squamous cell cancer yemapapu, uye hombe kenza yemapapu), zvichiunza kupenya kwetariro kuvarwere vane vari kurarama murima.Asi chiedza chekubuda kwemugero chinogona kunge chisisabatike nguva dzese, nekuti kusanzwa nemishonga kunowanzoita mushure memwedzi gumi kusvika gumi nemitanhatu yekurapwa.

Naizvozvo, kana tikakwanisa kubata nguva yekuvandudza kurapa kweGEF, edza kurapa kenza yemapapu kune inodzoreka uye inochengetedzwa zvirinani kana kutoderedza mhedzisiro yemishonga kuitira kuti varwere vave nemamiriro emuviri ari nani ekubata nawo. kenza, zvichida pane mukana wekuita kuti chiedza chehupenyu chipenye uye chipenye.

Vatsvagiri kubva kuDhipatimendi reOncology reYantai Hospital yeTraditional Chinese Medicine uye Medical College yeTibet University vakabatana vakaburitsa gwaro rekutsvagisa mu "Pharmaceutical Biology" mukupera kwa2020 izvo zvakaratidza kuburikidza nekuedza kwemhuka kuti kune yakajairika lung adenocarcinoma mune isiri diki. kenza yemapapu emasero, kushandiswa kwakabatanidzwa kweGanodermalucidumtriterpenoids (GLTs) uye GEF inogona kunyatso kuvharidzira kukura kwebundu uye kuderedza mhedzisiro yemishonga, ichipa chirongwa chitsva chakakosha kufunga nezvemaitiro ekurapa ane hukama.

Vatsvakurudzi vakatanga kuisa vanhu alveolar adenocarcinoma cell lines (A549 cell lines) pasi peganda rembeva dzine masoja ekudzivirira zvirwere.Mushure mokunge madhayamita emakumbo e-subcutaneous angangoita 6-8 mm, vakatanga kudyaGanoderma lucidumtriterpenoids (GLT, 1 g / kg / zuva), gefitinib (GEF, 15 mg / kg / zuva) kana kusanganiswa kwezvose kwemazuva 14, uye kuedza kwakaguma pazuva re15th.Zvakaitika kuti:

(1) Kuvandudza bundu kukura inhibition rate

GLTs uye GEF inogona kudzivisa kukura kwemapapu adenocarcinoma, asi kusanganiswa kwezviviri kune mhedzisiro iri nani (Mufananidzo 1 ~ 3).

Mufananidzo 1 Mapundu anotorwa mumapapu adenocarcinoma mbeva pakupera kwekuyedza

Mufananidzo 2 Shanduko mukukura kwebundu remapapu adenocarcinoma mbeva panguva yekuyedza

Mufananidzo 3 Tumor kukura inhibition rate yemapapu adenocarcinoma mbeva nenzira dzakasiyana dzekurapa.

2) Simbisa kuvharirwa kwe tumor angiogenesis uye kusimudzirwa kwekenza cell apoptosis

Mamota anofanirwa kugadzira midziyo mitsva kuti irambe ichikura.Naizvozvo, kuwanda kwemamicrovessels mu tumor tissues rave kiyi yakakosha pakukura kwakanaka kwemamota.Mufananidzo 4 (A) inoratidza kugoverwa kwemamicrovessels mune bundu tishu zvimedu zveboka rega rega.Mufananidzo 4 (B) inoratidza kuti kusanganiswa kweGLTs neGEF kune zviri nani inhibitory effect pane iyo miviri chete.

Mufananidzo 4 Tumor tissue zvikamu uye microvessel density yemapapu adenocarcinoma mice

Mune mamwe mazwi, kusanganiswa kweGLTs uye GEF kunogona kuvharira mamwe maturu emamota kubva pakuwana zvinovaka muviri uye kuita kuti mabundu anyanye kuoma kukura.Iyi nzira yekuita inobva pakusimbiswa kwekugadzirisa kwemaitiro ane chokuita nejeni uye kuvharwa kweprotein mumakumbo emasumbu, kusanganisira kurambidza "vascular endothelial growth factor receptor 2 (VEGFR2)" uye kukurudzira kugadzirwa kwe "Angiostatin" uye "endostatin".

Pamusoro pezvo, vaongorori vakacherekedzawo mune tumor tissue zvikamu zveboka rega rega rembeva kuti pasi pekuita kwakasanganiswa kweGLTs neGEF, secretion yeprotein (Bax) inosimudzira cancer cell apoptosis ichawedzera zvakanyanya apo secretion yeprotein (Bcl- 2) iyo inhibits apoptosis yekenza maseru ichaderera.Maseru emapapu adenocarcinoma anomhanyisa mukukura akananga kudivi reapoptosis mune iyi plus uye minus simba.

(3) Deredza migumisiro yemishonga

Lung adenocarcinoma mbeva dzaingorapwa neGEF dzaive nekuremerwa kwakanyanya;kune rumwe rutivi, kusanganiswa kweGLTs uye GEF kunogona kuchengetedza zvakanakisisa bodyweight yemapapu adenocarcinoma mice ── iri pedyo neyoyo yemakonzo (yakajairika boka rekutonga) (Mufananidzo 5).

Mukuwedzera, mbeva dzemapapu adenocarcinoma chete dzakarapwa neGEF dzakaratidza kushushikana, kuneta, kurara, kuderera kwekuita, kuderedza kudya uye ganda rakagomara.Nekudaro, aya mamiriro aive akareruka zvakanyanya kana asiri pachena muboka raibatwa nemusanganiswa weGLTs uye GEF.Zviripachena, GLTs inogona kugadzirisa yakashata mhedzisiro inokonzerwa neGEF.

Mufananidzo 5 Macurves ehuremu marekodhi uye shanduko mumapapu adenocarcinoma mbeva panguva yekuyedza

(4) Kuchengetedzwa kweGLTs

Kuti vaongorore kuchengetedzwa kweGLTs, vaongorori vakagadzira mitsara yakajairwa yealveolar epithelial cell mitsara BEAS-2B uye yemunhu alveolar adenocarcinoma cell mitsara A549 inoshandiswa mukuyedza mhuka zvakateerana neGLTs in vitro kwemaawa makumi mana nemasere.

Zvigumisiro zvinoratidzwa muMufananidzo 6. Apo GLTs (concentrations ye2.5 uye 5 mg / L) yakadzivisa mararamiro emapapu adenocarcinoma masero kusvika ku80-60%, masero akajairika akanga achiri mupenyu;kunyange pakakwirira, GLTs ichiri kubata zvakajeka masero ekenza uye masero akajairwa zvakasiyana, uye musiyano uyu wakatonyanya kukosha kupfuura GEF (Mufananidzo 7).

Mufananidzo 6 Iyo inhibitory mhedzisiro yeGLTs pakukura kwesero

Mufananidzo 7 Iyo inhibitory mhedzisiro yegefitinib pakukura kwesero

Zvinoenderana nekuongorora kwemuongorori, iyo IC50 kukosha kweGLTs pa48 h yekurapa kweA549 cell mitsara yaive 14.38 ± 0.29 mg/L nepo GLTs yairatidza ine simba shoma cytotoxic maitiro paBEAS-2B cell line ine IC50 kukosha kwe78.62 ± 2.53 mg/L, zvinoreva kuti kana maGLT achiuraya maseru ekenza, anogona kuramba akachengeteka kumasero akajairwa.

GLTs uye kurapa kwakanangwa kunofambidzana, zvichiita kuti kurapwa kuwedzere kuvimbisa.

Iyi tsvakiridzo yatiratidza:

Pasi pemamiriro akafanana ekuyedza, kutonga nemuromo kweGLTs kunogona kunge kusingave neyakafanana inhibitory mhedzisiro pamapapu emunhu adenocarcinoma tumors seGEF, asi GLTs haina mhedzisiro yeGEF.

Kana GLTs neGEF zvichishanda pamwe chete, hazvingogone kuwedzera inhibitory mhedzisiro pakukura kwebundu asi zvakare kuderedza mhedzisiro yegefitinib pahuremu, mweya, simba, kudya uye ganda.Iyi ndiyo inonzi "kuwedzera kushanda uye kuderedza chepfu".

Chikonzero nei GLTs inogona kuvandudza GEF inhibition yemapapu adenocarcinoma tumors ine chekuita ne "inhibiting tumor angiogenesis" uye "kusimudzira cancer cell apoptosis".

Kuti vaongorore gomarara remunhu mumhuka, vaongorori vakashandisa mbeva dzine immune immune system (kuti masero egomarara remunhu akure pamhando dzakasiyana).Naizvozvo, mhedzisiro yacho yaive mhedzisiro yeGLTs uye GEF pachayo pamasero egomarara.

Zvisinei, mukushandiswa kwechokwadi kwekurwisa kenza, basa rekudzivirira muviri rinofanira kubatanidzwa.Saka, kuwedzera kune GLTs uye GEF, kana "kuzvidzivirira kwakanaka" kwakawedzerwa, zvigumisiro zvichave zvakanyanya kubata maziso here?

Vatsvakurudzi havana kupa tsanangudzo yakawanda yeGLTs inoshandiswa mukuedza, asi maererano netsanangudzo yepepa, inofanira kunge yakasvibiswa yakasvibiswa yeGLTs dzakasiyana-siyana.Asi dhigirii inoshanda yegiremu imwe pakirogiramu yehuremu hwemuviri mumakonzo ndeyechokwadi yakawanda.Izvi zvinotiudza kuti maapplication anoshanda angangoda muyero wakakura kuti ushande.Kune rumwe rutivi, zvinotipawo tariro yokuti mune ramangwana zvingave zvichibvira kuwana zvinhu zvinokosha zvinogona kushanda pamwe chete kana kuti zviri nani pamadosi akaderera.

Chero zvazvingave, tsvakiridzo iyi yakaratidza kuti triterpenoids kubva kuGanoderma lucidum haingotadzisi kurapwa kwemishonga inowanzoshandiswa yekiriniki, asi zvakare ine mhedzisiro yakanaka ye "kuwedzera kugona uye kuderedza chepfu" zvichienderana nekuchengetedzwa kwakanyanya.
Kutsvanzvadzira mumugero werima kunoda mwenje wekenduru kuti utungamire nzira uye kuvhenekera.Kuenzaniswa ne "tariro" idzo dzisingasvikike kana kunetsa kugadzira-zhinji, kana "mabikirwo epachivande" ane zvisingazivikanwe masosi uye zvinongedzo,Ganoderma lucidumtriterpenoids, iyo inogona kuwanikwa chero yaunoda uye wakaunganidza kwenguva yakareba yekushandisa ruzivo, inofanirwa kuve yakakosha kuedza.

[Mabviro] Wei Liu, et al.Ganoderma triterpenoids attenuate bundu angiogenesis mukenza yemapapu bundu rinotakura nude mbeva.Nhoroondo ye Pharm Biol.2020: 58(1): 1061-1068.

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Nezve munyori/ Ms. Wu Tingyao
Wu Tingyao anga achizivisa nezvekutanga-ruoko ruzivo rweGanoderma kubvira 1999. Ndiye munyori weKuporesa neGanoderma(yakabudiswa muThe People's Medical Publishing House muna Kubvumbi 2017).
 
★ Chinyorwa ichi chakatsikiswa pasi pemvumo yakasarudzika yemunyori.★ Mabhuku ari pamusoro haagone kutsikiswa, kutorwa kana kushandiswa nedzimwe nzira pasina mvumo yemunyori.★ Nekutyorwa kwechirevo chiri pamusoro, munyori achatevera mabasa akakodzera emutemo.★ Manyoro ekutanga echinyorwa ichi akanyorwa neChinese naWu Tingyao uye akashandurirwa muchiRungu naAlfred Liu.Kana paine mutsauko pakati peshanduro (yeChirungu) neyekutanga (Chinese), iyo yekutanga Chinese ichakunda.Kana vaverengi vaine mibvunzo, tapota taura nemunyori wemabviro, Ms. Wu Tingyao.