Kaum Ib Hlis 8, 2020 / Medical College, Tibet University / Pharmaceutical Biology

Text/Wu Tingyao

Cov neeg mob qog noj ntshav tuaj yeem noj tauGanoderma lucidumthaum tau txais kev kho mob?Vam tias cov ntaub ntawv tshawb fawb hauv qab no tuaj yeem muab qee cov lus teb.

Gefitinib (GEF) yog ib lub hom phiaj tseem ceeb tshaj plaws rau kev kho mob ntawm cov mob qog noj ntshav siab thiab metastatic uas tsis yog cov qog nqaij hlav me me (xws li mob ntsws adenocarcinoma, squamous cell ntsws cancer, thiab mob ntsws cancer loj), ua rau muaj kev cia siab rau cov neeg mob. ciaj sia nyob hauv qhov tsaus ntuj.Tab sis lub teeb ntawm qhov tawm ntawm lub qhov yuav tsis nyob ib txwm nyob, vim hais tias cov tshuaj tiv thaiv zoo li loj hlob tom qab kaum mus rau kaum rau lub hlis ntawm kev kho mob.

Yog li ntawd, yog tias peb tuaj yeem txeeb lub sijhawm los txhim kho cov txiaj ntsig zoo ntawm GEF, sim kho mob qog noj ntshav kom tswj tau ntau dua thiab tswj tau zoo dua lossis txawm txo cov kev mob tshwm sim ntawm cov tshuaj kom cov neeg mob tuaj yeem muaj lub cev zoo dua los daws. mob qog noj ntshav, tej zaum muaj lub cib fim ua kom lub teeb ntawm lub neej ci ntsa iab thiab ci dua.

Cov kws tshawb fawb los ntawm Oncology Department ntawm Yantai Tsev Kho Mob ntawm Tsob Ntoo Suav Tshuaj thiab Tsev Kawm Qib Siab Kev Kho Mob ntawm Tibet University tau koom ua ke luam tawm tsab ntawv tshawb fawb hauv "Pharmaceutical Biology" thaum kawg ntawm 2020 uas tau ua pov thawj los ntawm cov tsiaj sim uas muaj feem ntau ntawm lub ntsws adenocarcinoma hauv cov khoom tsis me. cell ntsws cancer, kev siv ua ke ntawmGanodermalucidumtriterpenoids (GLTs) thiab GEF tuaj yeem cuam tshuam cov qog loj hlob zoo dua thiab txo cov kev mob tshwm sim ntawm cov tshuaj, muab cov phiaj xwm tshiab tsim nyog xav txog cov tswv yim kho mob.

Cov kws tshawb nrhiav thawj zaug tau cog rau tib neeg alveolar adenocarcinoma cell kab (A549 cell kab) nyob rau hauv daim tawv nqaij ntawm nas nrog kev tiv thaiv kab mob.Tom qab lub diameters ntawm cov qog subcutaneous yog kwv yees li 6-8 hli, lawv pib pubGanoderma lucidumtriterpenoids (GLT, 1 g / kg / hnub), gefitinib (GEF, 15 mg / kg / hnub) lossis kev sib xyaw ua ke ntawm ob qho tib si rau 14 hnub, thiab qhov kev sim tau xaus rau hnub 15.Nws muab tawm tias:

(1) Txhim kho qog kev loj hlob inhibition tus nqi

GLTs thiab GEF tuaj yeem cuam tshuam qhov kev loj hlob ntawm lub ntsws adenocarcinoma qog, tab sis kev sib xyaw ntawm ob muaj txiaj ntsig zoo dua (Daim duab 1 ~ 3).

Daim duab 1 Cov qog tshem tawm ntawm lub ntsws adenocarcinoma nas thaum kawg ntawm qhov kev sim

Daim duab 2 Kev hloov pauv hauv qog loj hlob ntawm lub ntsws adenocarcinoma nas thaum lub sijhawm sim

Daim duab 3 Cov qog loj hlob inhibition ntawm lub ntsws adenocarcinoma nas los ntawm ntau txoj kev kho mob

2) Txhim kho qhov inhibition ntawm qog angiogenesis thiab txhawb kev mob qog noj ntshav apoptosis

Cov qog yuav tsum tsim cov hlab ntsha tshiab thiaj li yuav loj hlob ntxiv.Yog li ntawd, qhov ntom ntawm microvessels hauv cov qog nqaij hlav tau dhau los ua tus yuam sij tseem ceeb rau kev loj hlob ntawm cov qog.Daim duab 4 (A) qhia txog kev faib cov microvessels hauv cov qog nqaij hlav ntawm txhua pab pawg.Daim duab 4 (B) qhia tias kev sib xyaw ntawm GLTs thiab GEF muaj kev cuam tshuam zoo dua li ob leeg ib leeg.

Daim duab 4 Cov qog nqaij hlav thiab microvessel ntom ntom ntawm lub ntsws adenocarcinoma nas

Hauv lwm lo lus, kev sib xyaw ua ke ntawm GLTs thiab GEF tuaj yeem thaiv cov qog nqaij hlav ntau dua los ntawm kev tau txais cov as-ham thiab ua rau cov qog loj hlob zuj zus.Cov txheej txheem ntawm kev ua haujlwm no los ntawm kev txhim kho kev tswj hwm ntawm cov noob caj noob ces thiab cov protein tso tawm hauv cov qog nqaij hlav, suav nrog inhibiting "vascular endothelial growth factor receptor 2 (VEGFR2)" thiab txhawb kev tsim cov "Angiostatin" thiab "endostatin".

Tsis tas li ntawd, cov kws tshawb fawb kuj tau pom nyob rau hauv cov qog nqaij hlav ntawm txhua pab pawg nas uas nyob rau hauv kev sib koom ua ke ntawm GLTs thiab GEF, qhov tso tawm ntawm cov protein (Bax) uas txhawb nqa cov qog nqaij hlav qog nqaij hlav cancer yuav nce ntxiv thaum lub zais ntawm cov protein (Bcl- 2) uas inhibits apoptosis ntawm cov qog nqaij hlav cancer yuav txo qis.Lub ntsws adenocarcinoma hlwb nrawm hauv kev txhim kho mus rau kev taw qhia ntawm apoptosis hauv qhov ntxiv thiab rho tawm quab yuam.

(3) Txo cov kev mob tshwm sim ntawm cov tshuaj

Mob ntsws adenocarcinoma nas uas tsuas yog kho nrog GEF muaj qhov hnyav tshaj plaws;ntawm qhov tod tes, kev sib xyaw ua ke ntawm GLTs thiab GEF tuaj yeem tswj hwm lub cev hnyav ntawm lub ntsws adenocarcinoma nas ── ze tshaj plaws ntawm cov nas ib txwm (ib pawg tswj hwm) (Daim duab 5).

Tsis tas li ntawd, mob ntsws adenocarcinoma nas tsuas yog kho nrog GEF pom kev ntxhov siab, qaug zog, tsaug zog, tsis ua haujlwm, txo qis qab los noj mov thiab dull daim tawv nqaij.Txawm li cas los xij, cov xwm txheej no tau sib dua lossis tsis pom tseeb hauv pab pawg kho nrog kev sib xyaw ntawm GLTs thiab GEF.Pom tseeb, GLTs tuaj yeem kho qhov tsis zoo tshwm sim los ntawm GEF.

Daim duab 5 Curves ntawm qhov hnyav cov ntaub ntawv thiab cov kev hloov hauv lub ntsws adenocarcinoma nas thaum lub sij hawm sim

(4) Kev nyab xeeb ntawm GLTs

Txhawm rau ntsuas kev nyab xeeb ntawm GLTs, cov kws tshawb fawb tau coj tib neeg alveolar epithelial cell kab BEAS-2B thiab tib neeg alveolar adenocarcinoma cell kab A549 siv hauv kev sim tsiaj raws li GLTs hauv vitro rau 48 teev.

Cov txiaj ntsig tau pom nyob rau hauv daim duab 6. Thaum GLTs (concentrations ntawm 2.5 thiab 5 mg / L) inhibited qhov ciaj sia taus ntawm lub ntsws adenocarcinoma hlwb mus rau 80-60%, ib txwm hlwb tseem ciaj sia;Txawm tias nyob rau hauv siab, GLTs tseem qhia meej kho cov qog nqaij hlav cancer thiab cov hlwb ib txwm sib txawv, thiab qhov kev sib txawv no tseem ceeb dua li GEF (Daim duab 7).

Daim duab 6 Cov nyhuv inhibitory ntawm GLTs ntawm kev loj hlob ntawm tes

Daim duab 7 Cov nyhuv inhibitory ntawm gefitinib ntawm kev loj hlob ntawm tes

Raws li tus kws tshawb fawb soj ntsuam, IC50 qhov tseem ceeb ntawm GLTs ntawm 48 h ntawm kev kho mob rau A549 kab ntawm tes yog 14.38 ± 0.29 mg / L thaum GLTs pom muaj zog cytotoxic ntau dua ntawm BEAS-2B cell kab nrog IC50 tus nqi ntawm 78.62. ± 2.53 mg / L, uas txhais tau hais tias thaum GLTs tuag rau cov qog nqaij hlav cancer, lawv tseem tuaj yeem ua kom muaj kev nyab xeeb siab rau cov hlwb qub.

GLTs thiab cov phiaj xwm kev kho mob sib koom tes, ua rau kev kho mob zoo dua.

Daim ntawv qhia kev tshawb fawb no tau qhia peb:

Raws li tib txoj kev sim, kev tswj hwm qhov ncauj ntawm GLTs yuav tsis muaj qhov cuam tshuam tib yam rau tib neeg lub ntsws adenocarcinoma qog raws li GEF, tab sis GLTs tsis muaj kev phiv los ntawm GEF.

Thaum GLTs thiab GEF ua haujlwm ua ke, lawv tuaj yeem tsis tsuas yog nce cov nyhuv inhibitory ntawm cov qog loj hlob tab sis kuj txo cov gefitinib cov teebmeem ntawm qhov hnyav, tus ntsuj plig, qhov tseem ceeb, qab los noj mov thiab tawv nqaij.Qhov no yog qhov hu ua "ua kom muaj txiaj ntsig thiab txo cov tshuaj lom".

Yog vim li cas vim li cas GLTs tuaj yeem txhim kho GEF qhov inhibition ntawm lub ntsws adenocarcinoma qog muaj feem xyuam rau "inhibiting qog angiogenesis" thiab "txhim kho mob qog noj ntshav apoptosis".

Txhawm rau ntsuas tib neeg mob qog noj ntshav hauv cov tsiaj, cov kws tshawb fawb tau siv cov nas uas muaj lub cev tsis muaj zog (kom tib neeg cov qog nqaij hlav cancer tuaj yeem loj hlob ntawm ntau hom).Yog li, cov txiaj ntsig tau pib ua rau GLTs thiab GEF nws tus kheej ntawm cov qog nqaij hlav cancer.

Txawm li cas los xij, hauv kev siv tiag tiag ntawm kev tiv thaiv kab mob qog noj ntshav, kev ua haujlwm ntawm lub cev tiv thaiv kab mob yuav tsum koom nrog.Yog li, ntxiv rau GLTs thiab GEF, yog tias "kev tiv thaiv zoo" ntxiv, cov txiaj ntsig puas yuav pom ntau dua?

Cov kws tshawb fawb tsis tau piav qhia ntau txog GLTs siv hauv kev sim, tab sis raws li cov lus piav qhia ntawm daim ntawv, nws yuav tsum yog cov khoom siv raw ntawm ntau hom GLTs.Tab sis cov koob tshuaj zoo ntawm ib gram ib kilogram ntawm lub cev hnyav hauv nas yog qhov ntau heev.Qhov no qhia peb tias cov kev siv tswv yim yuav xav tau ntau npaum li cas thiaj li ua tau zoo.Ntawm qhov tod tes, nws kuj ua rau peb cia siab tias yav tom ntej nws yuav tuaj yeem nrhiav cov khoom xyaw tseem ceeb uas tuaj yeem ua haujlwm zoo lossis zoo dua ntawm cov tshuaj qis dua.

Txawm li cas los xij, tsawg kawg qhov kev tshawb fawb no tau pom tias triterpenoids los ntawm Ganoderma lucidum tsis tsuas yog tsis cuam tshuam kev kho mob ntawm cov tshuaj siv tshuaj kho mob uas nquag siv, tab sis kuj muaj cov txiaj ntsig zoo ntawm "ua kom muaj txiaj ntsig thiab txo cov tshuaj lom" raws li kev nyab xeeb ntau.
Groping nyob rau hauv qhov tsaus ntuj yuav tsum tau ntau dua tswm ciab coj txoj kev thiab illumination.Piv nrog rau cov "kev cia siab" uas tsis ncav cuag lossis nyuaj rau kev tsim khoom loj, lossis "cov zaub mov txawv" uas tsis paub qhov chaw thiab cov khoom xyaw,Ganoderma lucidumtriterpenoids, uas tuaj yeem tau ntev li ntev tau raws li koj xav tau thiab tau txais kev paub txog kev siv sijhawm ntev, yuav tsum tsim nyog sim.

[Source] Wei Liu, et al.Ganoderma triterpenoids attenuate qog angiogenesis nyob rau hauv lub ntsws cancer qog-cov kabmob liab qab nas.Pharm Biol.2020: 58(1): 1061-1068.

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Hais txog tus sau/ Ms. Wu Tingyao
Wu Tingyao tau tshaj tawm cov ntaub ntawv Ganoderma thawj zaug txij li xyoo 1999. Nws yog tus sau ntawmKho nrog Ganoderma(tshaj tawm hauv Lub Tsev Kho Mob Tib Neeg Lub Plaub Hlis 2017).
 
★ Kab lus no tau luam tawm raws li kev tso cai tshwj xeeb ntawm tus sau.★ Cov haujlwm saum toj no tsis tuaj yeem muab luam tawm, txiav tawm lossis siv lwm txoj hauv kev yam tsis muaj kev tso cai los ntawm tus sau.★ Rau kev ua txhaum ntawm cov lus hais saum toj no, tus sau yuav ua raws li cov luag haujlwm raug cai.★ Cov ntawv qub ntawm kab lus no tau sau ua lus Suav los ntawm Wu Tingyao thiab txhais ua lus Askiv los ntawm Alfred Liu.Yog tias muaj qhov tsis sib xws ntawm kev txhais lus (English) thiab tus thawj (Suav), tus thawj Suav yuav yeej.Yog tias cov nyeem muaj lus nug, thov hu rau tus thawj kws sau ntawv, Ms. Wu Tingyao.