NgoJanuwari 2020/iYunivesithi yasePeking/Acta Pharmacologica Sinica

Umbhalo/ Wu Tingyao

Iqela elikhokelwa nguNjingalwazi u-Baoxue Yang, usihlalo weSebe le-Pharmacology, iYunivesithi yasePeking, lipapashe amanqaku amabini kwi-Acta Pharmacologica Sinica ekuqaleni kuka-2020, eqinisekisa ukubaI-Ganoderma lucidumI-triterpenes inokulibazisa ukuqhubeka kwe-renal fibrosis kunye nesifo sezintso ze-polycystic, kwaye ezona nxalenye zazo zisebenzayo yi-ganoderic acid A.

Iasidi yeGanoderic ilibazisa ukuqhubela phambili kwe-renal fibrosis.

Abaphandi babophe i-ureter kwelinye icala le mouse.Kwiintsuku ezilishumi elinesine kamva, impuku yayiza kuba ne-renal fibrosis ngenxa yokuphazamiseka kokuchama kunye nokubuyela umva komchamo.Ngelo xesha, igazi layo i-urea nitrogen (BUN) kunye ne-creatinine (Cr) nayo iya kwanda, ebonisa ukungasebenzi kakuhle kwezintso.

Nangona kunjalo, ukuba i-ganoderic acid inikezelwa kwi-dose yemihla ngemihla ye-50 mg / kg nge-injection ye-intraperitoneal ngokukhawuleza emva kokuhlanganiswa kwe-ureter, iqondo le-renal fibrosis okanye ukukhubazeka komsebenzi wezintso kuya kuncitshiswa kakhulu emva kweentsuku ze-14.

Uhlalutyo olongezelelweyo lwendlela yokusebenza ehambelanayo lubonisa ukuba i-ganoderic acid inokuthintela ukuqhubeka kwe-renal fibrosis ukusuka kubuncinci imiba emibini:

Okokuqala, i-ganoderic acids ikhusela iiseli eziqhelekileyo ze-renal tubular epithelial ekuguquleni kwiiseli ze-mesenchymal ezikhupha izinto ezinxulumene ne-fibrosis (le nkqubo ibizwa ngokuba yi-epithelial-to-mesenchymal transition, EMT);okwesibini, i-ganoderic acids inokunciphisa ukubonakaliswa kwe-fibronectin kunye nezinye izinto ezinxulumene ne-fibrosis.

Njengeyona triterpenoid ininzi kakhuluI-Ganoderma lucidum, iGanoderic acid ineentlobo ezininzi.Ukuze kuqinisekiswe ukuba yeyiphi i-ganoderic acid eyenza umphumo wokukhusela izintso ezikhankanywe ngasentla, abaphandi bahlakulela i-ganoderic acids ephambili A, B, kunye ne-C2 kunye nemigca yeseli ye-renal tubular epithelial kwi-concentration ye-100 μg / mL.Kwangaxeshanye, into yokukhula kwe-TGF-β1, eyimfuneko ekuqhubekeni kwe-fibrosis, yongezwa ukuze kufakwe iiseli ukuba zikhuphe iiproteni ezinxulumene ne-fibrosis.

Iziphumo zibonisa ukuba i-ganoderic acid A inefuthe elihle kakhulu ekuvimbeleni ukukhutshwa kweeprotheni ezinxulumene ne-fibrosis kwiiseli, kwaye umphumo wayo unamandla ngakumbi kunomxube we-ganoderic acid.Ngoko ke, abaphandi bakholelwa ukubaI-Ganoderma lucidumngumthombo osebenzayo wokunciphisa i-fibrosis yezintso.Kubaluleke kakhulu ukuba i-ganoderic acid A ayinayo impembelelo eyityhefu kwiiseli zezintso kwaye ayiyi kubulala okanye yenzakalise iiseli zezintso.

Iiasidi zeGanoderic zilibazisa ukuqhubela phambili kwesifo sezintso ze-polycystic.

Ngokungafaniyo ne-renal fibrosis, ebangelwa kakhulu zizinto zangaphandle ezifana nezifo kunye neziyobisi, isifo se-polycystic kidney sibangelwa ukuguqulwa kofuzo kwi-chromosome.Ii-vesicles kumacala omabini ezintso ziya kuthi ngokuthe ngcembe zibe zikhulu kwaye zibe ninzi ngakumbi ukuze ucinezele kwizicubu eziqhelekileyo zezintso kunye nokuphazamiseka kokusebenza kwezintso.

Ngaphambili, iqela likaBaoxue Yang likubonakalisile okoI-GanodermalucidumI-triterpenes inokulibazisa ukuqhubeka kwesifo sezintso ze-polycystic kunye nokukhusela ukusebenza kwezintso.Nangona kunjalo, iI-GanodermalucidumI-triterpenes esetyenziswe kuvavanyo ubuncinane iquka i-ganoderic acids A, B, C2, D, F, G, T, DM kunye ne-ganoderenic acids A, B, D, kunye ne-F.

Ukufumana ezona zithako zisebenzayo, abaphandi baphonononge iintlobo ezili-12 ze-triterpenes nganye nganye ngovavanyo lwe-vitro kwaye bafumanisa ukuba akukho nanye kuzo echaphazela ukusinda kweeseli zezintso kodwa banomahluko omkhulu ekuthinteleni ukukhula kwe-vesicle.Phakathi kwabo, i-ganoderic acid A inefuthe elihle kakhulu.

Ngaphaya koko, i-ganoderic acid A yakhuliswa kwi-vitro kunye nezintso zeempuku ze-embryonic kunye neearhente ezikhuthaza ukwenziwa kwe-vesicle.Ngenxa yoko, i-ganoderic acid A isenokuthi ithintele inani kunye nobukhulu be-vesicles ngaphandle kokuchaphazela ukukhula kwezintso.Umthamo wayo osebenzayo wawuyi-100μg / mL, efanayo nedosi ye-triterpenes esetyenziswe kwizilingo zangaphambili.

Uvavanyo lwezilwanyana luye lwafumanisa ukuba i-injection subcutaneous ye-50 mg / kg ye-ganoderic acid A kwiigundane ezizalwa ngokukhawuleza ezine-polycystic kidney disease yonke imihla, emva kweentsuku ezine zonyango, kunokuphucula ukudumba kwezintso ngaphandle kokuchaphazela ubunzima besibindi kunye nobunzima bomzimba.Kwakhona kunciphisa umthamo kunye nenani le-renal vesicles, ukwenzela ukuba indawo yokusabalalisa i-renal vesicles iyancitshiswa malunga ne-40% xa kuthelekiswa neqela lokulawula ngaphandle kwe-ganoderic acid A yokukhusela.

Ukusukela ukuba ithamo elisebenzayo le-ganoderic acid A kuvavanyo yayisisisinye kwisine sovavanyo olufanayoGi-anodermalucidumtriterpenes, kuboniswa ukuba i-ganoderic acid A ngokwenene iyinxalenye ephambiliGi-anodermalucidumtriterpenes ukulibazisa ukuqhubela phambili kwesifo sezintso ze-polycystic.Ukusebenzisa idosi efanayo ye-ganoderic acid A kwiigundane eziqhelekileyo ezisandul 'ukuzalwa akuzange kuchaphazele ubungakanani bezintso zabo, kubonisa ukuba i-ganoderic acid A inezinga elithile lokhuseleko.

Ukusuka kwi-renal fibrosis ukuya kwi-renal failure, kunokuthiwa ukuba isifo sezintso esingapheliyo esibangelwa yizizathu ezahlukahlukeneyo (ezifana nesifo sikashukela) ngokuqinisekileyo siya kuhamba kwindlela yokungabuyi.

Kwizigulane ezinesifo sezintso ze-polycystic, isantya sokuhla komsebenzi wezintso sinokukhawuleza.Ngokwezibalo, malunga nesiqingatha sezigulane ezine-polycystic isifo sezintso ziya kuqhubela phambili ekungaphumeleli kwezintso malunga neminyaka engama-60 kwaye zifuna i-dialysis yobomi bonke.

Kungakhathaliseki ukuba i-pathogenic factor ifunyenwe okanye i-congenital, akulula "ukuguqula umsebenzi wezintso"!Nangona kunjalo, ukuba izinga lokuwohloka kwezintso linokuthotywa ukuze lilingane nobude bobomi, kunokwenzeka ukwenza ubomi obugulayo bungabi nathemba kwaye bubonakale ngakumbi.

Ngovavanyo lweeseli kunye nezilwanyana, iqela lophando lika-Baoxue Yang libonise ukuba i-Ganoderic acid A, ebangela umlinganiselo ophezuluI-Ganoderma lucidumtriterpenes, yinxalenye yesalathisi seI-Ganoderma lucidumukukhusela intso.

Esi siphumo sophando sigxininisa ukuba uphando lwezenzululwazi lweI-Ganoderma lucidumyomelele kangangokuba inokukuxelela ukuba yeyiphi isithako iziphumo zeI-Ganoderma lucidumubukhulu becala zivela endaweni yokuzoba nje ipayi yentelekelelo yentelekelelo yakho.Kakade ke, akuthethi ukuba kuphela i-ganoderic acid A inokukhusela intso.Enyanisweni, ezinye izithakoI-Ganoderma lucidumngokuqinisekileyo ziluncedo kwizintso.

Umzekelo, elinye iphepha elipapashwe liqela likaBaoxue Yang ngesihloko sokukhusela izintso lathiI-Ganoderma lucidumI-polysaccharide extract inokunciphisa umonakalo oxidative kwizicubu zezintso ngokusebenzisa i-antioxidant effect.I-“I-Ganoderma lucidumi-triterpenes iyonke”, equlethe ii-triterpenoids ezahlukeneyo ezifana ne-ganoderic acids, i-ganoderenic acids kunye ne-ganoderiols, zisebenza kunye ukulibazisa ukuqhubeka kwe-renal fibrosis kunye nesifo sezintso ze-polycystic, nto leyo eyothusa izazinzulu.

Ngaphezu koko, imfuneko yokukhusela intso ayisonjululwa ngokukhusela intso kuphela.Ezinye izinto ezinjengokulawula ukhuselo lomzimba, ukuphucula imigangatho emithathu, ukulinganisa i-endocrine, ukuthomalalisa imithambo-luvo kunye nokuncedisa ukulala ngokuqinisekileyo luncedo ekukhuseleni izintso.Ezi ntlonipho azinakusonjululwa ngokupheleleyo nge-ganoderic acid A yodwa.

Ukuxabiseka kweI-Ganoderma lucidumilele kwizithako zayo ezahlukahlukeneyo kunye nemisebenzi eguquguqukayo, enokuthi ilungelelanise enye nenye ukuvelisa eyona ibhalansi yomzimba.Ngamanye amazwi, ukuba i-ganoderic acid A iyanqongophala, umsebenzi wokukhusela izintso uya kuswela amandla amaninzi okulwa njengeqela eliswele abadlali abaphambili.

I-Ganoderma lucidumkunye ne-ganoderic acid A ifaneleke ngakumbi kwizinto esizilindeleyo ngenxa yempembelelo yayo engcono yokukhusela izintso.

[Umthombo weDatha]

1. Geng XQ, et al.I-Ganoderic acid ithintela i-renal fibrosis ngokucinezela i-TGF-β/Smad kunye ne-MAPK iindlela zokubonisa.Acta Pharmacol Sin.2020, 41: 670-677.doi: 10.1038/s41401-019-0324-7.

2. UMeng J, et al.I-Ganoderic acid A sisithako esisebenzayo se-Ganoderma triterpenes ekuthinteleni ukukhula kwe-cyst ye-renal kwisifo sezintso ze-polycystic.Acta Pharmacol Sin.2020, 41: 782-790.doi: 10.1038/s41401-019-0329-2.

3. USu L, kunye nabanye.I-Ganoderma triterpenes ibuyisela uphuhliso lwe-cyst ye-renal ngokuthoba umqondiso we-Ras / MAPK kunye nokukhuthaza ukuhlukana kweeseli.Izintso Int.2017 Dec;92(6): 1404-1418.doi: 10.1016/j.kint.2017.04.013.

4. Zhong D, kunye nabanye.I-Ganoderma lucidum polysaccharide peptide ikhusela ukwenzakala kwe-ischemia ye-renal reperfusion ngokuchasana noxinzelelo lwe-oxidative.Sci Rep. 2015 Nov 25;5: 16910. doi: 10.1038/srep16910.

ISIPHELO

Malunga nombhali/ Nksz. Wu Tingyao
UWu Tingyao unike ingxelo okokuqalaI-Ganoderma lucidumulwazi ukususela ngo-1999. Ungumbhali weUkunyanga ngeGanoderma(ipapashwe kwiNdlu yePeople's Medical Publishing House ngo-Epreli 2017).
 
★ Eli nqaku lipapashwa phantsi kogunyaziso olukhethekileyo lombhali ★ Le misebenzi ingentla ayinakuphinda kuveliswe, icatshulwe okanye isetyenziswe ngezinye iindlela ngaphandle kokugunyaziswa ngumbhali ★ Ukwaphulwa kwale nkcazo ingasentla, umbhali uya kuqhuba uxanduva lwakhe lomthetho olunxulumeneyo ★ Eyokuqala umbhalo weli nqaku ubhalwe ngesiTshayina nguWu Tingyao kwaye waguqulelwa kwisiNgesi nguAlfred Liu.Ukuba kukho nakuphi na ukungangqinelani phakathi kwenguqulelo (isiNgesi) kunye neyoqobo (isiTshayina), isiTshayina sokuqala siya koyisa.Ukuba abafundi banayo nayiphi na imibuzo, nceda uqhagamshelane nombhali wokuqala, uNks. Wu Tingyao.