January 10, 2017 / Univesithi ea Tongji, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, joalo-joalo / Stem Cell Reports

Mongolo/Wu Tingyao

“Lebala hore na u mang le hore na ke mang” ho ka boleloa hore ke letšoao le tloaelehileng haholo la lefu la Alzheimer.Lebaka la ho lebala, kapa ho se khone ho hopola liketsahalo tsa morao-rao, ke hore lisele tsa methapo tse laolang tšebetso ea kelello li shoa hanyane ka hanyane ha lilemo li ntse li feta, e leng se etsang hore motho e moholo akelello boemotsoela pele ho senyeha.

Ha ba tobane le lefu lena la Alzheimer le ntseng le ata, bo-rasaense ba sebetsa ka thata ho ithuta mekhoa ea phekolo e ka khonehang.Batho ba bang ba tsepamisa maikutlo ho molato o bakang lefu la lisele tsa methapo, ho leka ho fokotsa tlhahiso ea protheine ea beta-amyloid;ba bang ba ikemiselitse ho khothalletsa ho tsosolosoa ha methapo ea kutlo, ba tšepile ho koala sekheo sa tšenyo ea lisele tsa kutlo, eo mohlomong e leng khopolo ea “ho e lokisa haeba e le sieo.”

Bokong ba liphoofolo tse anyesang tse hōlileng tsebong, ho hlile ho na le libaka tse peli tse tsoelang pele ho hlahisa lisele tse ncha tsa methapo, e 'ngoe ea tsona e leng ka har'a gyrus ea hippocampal.Lisele tsena tsa methapo e ikatisang li bitsoa "neural progenitor cells".Lisele tse sa tsoa tsoaloa ho tsoa ho tsona li tla eketsoa ho li-circuits tsa mantlha tsa methapo ho thusa ho ithuta mahlale a macha le ho theha mehopolo e mecha.

Leha ho le joalo, ho ka hlokomeloa ho batho kapa litoeba hore lefu la Alzheimer le ka sitisa ho ata ha lisele tsa methapo ea kutlo.Matsatsing ana, bopaki bo ntseng bo eketseha bo bontša hore ho khothalletsa ho ata ha lisele tsa methapo ea kutlo ho ka fokotsa ho senyeha ha kelello ho bakoang ke lefu la Alzheimer 'me e ka ba leano le ka khonehang la ho phekola lefu la Alzheimer's.

Ka Pherekhong 2017, phuputso e phatlalalitsoeng ka kopanelo ho "Stem Cell Reports" ke Univesithi ea Tongji, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, joalo-joalo, e pakile hore li-polysaccharides kapa li-extracts tsa metsi ho tsoa.Ganoderma lucidum (Reishi mushroom, Lingzhi) e ka fokotsa ho senyeha ha kelello ho bakoang ke lefu la Alzheimer, ho fokotsa ho kenngoa ha amyloid-β (Aβ) bokong, le ho khothalletsa ho tsosolosoa ha lisele tsa neural precursor gyrus ea hippocampal.Mokhoa oa ho qetela oa ts'ebetso o kanna oa amana le ts'ebetso ea receptor e bitsoang FGFR1 ho lisele tsa neural precursor ka lebaka la taolo eaGanoderma lucidum.

Litoeba tsa Alzheimer tse jangGanoderma lucidumhopola hantle.

Liteko tsa liphoofolo thutong ena li sebelisitse litoeba tsa APP/PS1 tsa likhoeli tse 5 ho isa ho tse 6-ke hore, ts'ebeliso ea theknoloji ea phetisetso ea liphatsa tsa lefutso ho fetisetsa liphatsa tsa lefutso tsa motho tse fetotsoeng APP le PS1 (e ka bakang lefu la Alzheimer's pele ho qaleha). litoeba tse sa tsoa tsoaloa bakeng sa ho hlahisa liphatsa tsa lefutso ka katleho.Sena se tla etsa hore boko ba litoeba bo qale ho hlahisa amyloid-β (Aβ) ho tloha bonyenyaneng (ka mor'a likhoeli tsa 2), 'me ha li hōla ho fihlela likhoeling tsa 5-6, butle-butle li tla ba le bothata ba ho lemoha sebaka le mohopolo. .

Ka mantsoe a mang, litoeba tse sebelisitsoeng tekong li ne li se li ntse li e-na le matšoao a pele a lefu la Alzheimer's.Bafuputsi ba ile ba fepa litoeba tse joalo tsa Alzheimer ka GLP (polysaccharides e hloekileng e arohaneng le eonaGanoderma lucidumphofo ea spore e nang le boima ba molek'hule ea 15 kD) ka tekanyo ea letsatsi le letsatsi ea 30 mg / kg (ke hore, 30 mg ka kilogram ea boima ba 'mele ka letsatsi) bakeng sa matsatsi a 90 a latellanang.

Joale, bafuputsi ba ile ba qeta matsatsi a mang a 12 ba lekola bokhoni ba kelello ba litoeba tsa Morris water maze (MWM) mme ba li bapisa le tsa litoeba tse nang le lefu la Alzheimer tse neng li sa fumana kalafo le tsa litoeba tse tloaelehileng.

Litoeba li na le lehloeo la tlhaho ho metsi.Ha li kenngoa ka metsing, li tla leka ho fumana sebaka se omileng sa ho phomola."Teko ea Morris Water Maze" e sebelisa tlhaho ea bona ho theha sethala sa ho phomola sebakeng se tsitsitseng letamong le leholo le chitja.Kaha sethala se patiloe ka tlas'a metsi, litoeba li tlameha ho se fumana feela ka ho ithuta le ho hopola.Ka lebaka leo, bafuputsi ba ne ba ka ahlola hore na litoeba li ntse li fumana dumber kapa bohlale nakong eo litoeba li fumanang sethala, sebaka seo li sesang le tsela eo li e nkileng.

Ho ile ha fumanoa hore ho ne ho se na phapang e khōlō ka lebelo la ho sesa la litoeba sehlopheng ka seng.Empa ha ho bapisoa le litoeba tse tloaelehileng, litoeba tsa Alzheimer tse neng li sa fumana phekolo li ile tsa tlameha ho qeta nako e ngata le ho sesa sebaka se selelele ho fumana sethala ka tsela e sa tsitsang joalokaha eka ke lehlohonolo, ho bontša hore mohopolo oa bona oa sebaka o ne o senyehile haholo.

Ho fapana le hoo, litoeba tsa Alzheimer li fepehile kaReishi li-mushroompolysaccharides kapaGanoderma lucidummetsi a nkiloeng a ile a fumana sethala ka potlako, 'me pele a fumana sethala, ba ile ba lelera haholo-holo sebakeng seo (quadrant) moo sethala se neng se le teng, joalokaha eka ba tseba sebaka se hakanyetsoang sa sethala, se bontšang hore tšenyo ea boko ba bona ha e kholo hakaalo.【Setšoantšo sa 1, Setšoantšo sa 2】

Ho phaella moo, bafuputsi ba ile ba boela ba hlokomela tekong e 'ngoe ea hore bakeng sa lintsintsi tsa litholoana tse hlahisang palo e kholo ea amyloid-β (Aβ) bokong ba tsona (hape ka mekhoa ea ho fetisetsa liphatsa tsa lefutso ho theha mehlala ea liteko),Ganoderma lucidummetsi a nkiloeng ha a ka ntlafatsa feela temoho ea sebaka le bokhoni ba ho hopola lintsintsi tsa litholoana empa hape a lelefatsa bophelo ba lintsintsi tsa litholoana.

Bafuputsi le bona ba ile ba sebelisaGanoderma lucidummetsi (300mg/kg ka letsatsi) litekong tse boletsoeng ka holimo tsa liphoofolo mme a fumana hore a ka fokotsa ts'ebello ea kelello ea sebaka e bakoang ke lefu la Alzheimer joalo ka ha ho boletsoe ka holimo.Ganoderma lucidumli-polysaccharides (GLP).

Sebelisa "Morris Water Maze Test" ho lekola bokhoni ba memori ea sebaka sa litoeba

[Setšoantšo se leqepheng la 1] Litsela tsa ho sesa tsa litoeba sehlopheng ka seng.Boputsoa ke letamo, bosoeu ke boemo ba sethala, 'me bofubelu ke tsela ea ho sesa.

[Setšoantšo sa 2] Karolelano ea nako e hlokahalang bakeng sa sehlopha ka seng sa litoeba ho fumana sethala sa ho phomola ka letsatsi la 7th la tlhahlobo ea maze ea metsi ea Morris

(Litlaleho tsa Mohloli/Stem Cell. 2017 Jan 10;8(1):84-94.)

Lingzhie khothalletsa ho ata ha lisele tsa neural precursor gyrus ea hippocampal.

Kamora tlhahlobo ea matsatsi a 12 ea maze ea metsi, bafuputsi ba ile ba sekaseka boko ba litoeba mme ba fumana horeGanoderma lucidumpolysaccharides leGanoderma lucidumlihlahisoa tsa metsi ka bobeli li khothalletsa ho tsosolosoa ha lisele tsa methapo ho hippocampal gyrus le ho fokotsa li-amyloid-β deposition.

Ho ile ha boela ha netefatsoa hore lisele tsa methapo e sa tsoa tsoaloa ka har'a hippocampus gyrus haholo-holo ke lisele tsa neural precursor.LeGanoderma lucidume sebetsa hantle bakeng sa litoeba tsa lefu la Alzheimer.Ho fepa litoeba tse tloaelehileng tse nyane leGanoderma lucidumpolysaccharides (GLP) ka tekanyo ea letsatsi le letsatsi ea 30 mg / kg bakeng sa matsatsi a 14 e ka boela ea khothalletsa ho ata ha lisele tsa neural precursor gyrus ea hippocampal.

Liteko tsa in vitro li boetse li netefalitse hore bakeng sa lisele tsa neural precursor tse arohaneng le hippocampal gyrus ea litoeba tse tloaelehileng tsa batho ba baholo kapa litoeba tsa Alzheimer kapa lisele tsa neural precursor tse tsoang liseleng tsa motho,Ganoderma lucidumli-polysaccharides li ka khothalletsa lisele tsena tsa pele hore li ata, 'me lisele tse sa tsoa hlahisoa li boloka litšobotsi tsa pele tsa lisele tsa neural precursor, ke hore, li ka etsa ho ata le ho inchafatsa.

Tlhahlobo e eketsehileng e bontšitse seoGanoderma lucidumpolysaccharides (GLP) e ka khothaletsa neurogeneis haholo hobane e ka matlafatsa mokelikeli o bitsoang "FGFR1" (eseng EGFR receptor) liseleng tsa neural precursor, e leng ho etsang hore e be kotsing ea ho susumetsoa ke "nerve growth factor bFGF", e romellang boitsebiso bo eketsehileng ba "sele". ho ata” liseleng tsa methapo ea kutlo, ebe ho hlaha lisele tse ling tse ncha tsa methapo.

Kaha lisele tsa methapo tse sa tsoa tsoaloa li ka tsoela pele ho ikopanya le li-circuits tse teng tsa neural ho sebetsa ka mor'a hore li fallele sebakeng sa boko se li hlokang, sena se lokela ho fokotsa mefuta e mengata ea mathata a kelello a bakoang ke lefu la methapo ea kutlo lefung la Alzheimer's.

Karolo e nang le mefuta e mengata eaGanoderma lucidume fokotsa lebelo la ho lebala.

Liphetho tsa lipatlisiso tse ka holimo li re lumella ho bona phello e sireletsang eaGanoderma lucidumliseleng tsa methapo.Ntle le ts'ebetso ea eona ea anti-inflammatory, anti-oxidant, anti-apoptotic, anti-β-amyloid le litlamorao tse ling tse tsejoang nakong e fetileng,Khanodermalucidume ka boela ea khothalletsa neurogenesis.Bakeng sa litoeba tsa Alzheimer tse nang le bofokoli bo tšoanang ba liphatsa tsa lefutso 'me li le matšoao a tšoanang, ke ka lebaka lena ho teba ha matšoao a lefu lena ho fapaneng haholo pakeng tsa ba jang.Ganoderma lucidumle ba sa jengGanoderma lucidum.

Ganoderma lucidume kanna ea se khone ho khutlisa ts'ebetso ea mohopolo ka botlalo ho bakuli ba Alzheimer, empa mekhoa ea eona e fapaneng ea ts'ebetso e ka liehisa ho senyeha ha lefu la Alzheimer's.Hafeela mokuli a ntse a ikhopotsa eena le ba bang bophelo bohle ba hae, lefu la Alzheimer le ka ’na la se ke la ba le tšabehang hakaalo.

[Mohloli] Huang S, et al.Li-polysaccharides tse tsoang ho Ganoderma lucidum li Khothaletsa Mosebetsi oa Tsebo le Neural Progenitor Proliferation ho Mouse Model oa Lefu la Alzheimer.Litlaleho tsa Stem Cell.2017 Jan 10;8(1):84-94.doi: 10.1016/j.stemcr.2016.12.007.

QETA

Mabapi le mongoli/ Mme Wu Tingyao

Wu Tingyao esale a tlaleha ka litaba tsa Ganoderma tsa pele ho tloha 1999. Ke eena mongoli oaHo folisa ka Ganoderma(e hatisitsoe ho The People's Medical Publishing House ka April 2017).

★ Sengoliloeng sena se hatisitsoe tlas'a tumello e khethehileng ea mongoli.★ Libuka tse ka holimo li ke ke tsa hlahisoa, tsa qotsoa kapa tsa sebelisoa ka litsela tse ling ntle le tumello ea mongoli.★ Bakeng sa tlōlo ea polelo e ka holimo, mongoli o tla phehella boikarabelo bo nepahetseng ba molao.★ Sengoloa sa mantlha sa sengoloa sena se ngotsoe ka Sechaena ke Wu Tingyao mme se fetoleloa ho Senyesemane ke Alfred Liu.Haeba ho na le phapang lipakeng tsa phetolelo (Senyesemane) le ea mantlha (Sechaena), Sechaena sa mantlha se tla ba teng.Haeba babali ba na le lipotso, ka kopo ikopanye le sengoli sa mantlha, Mme Wu Tingyao.