Lub ib hlis ntuj 10, 2017 / Tongji University, Shanghai lub koom haum ntawm Materia Medica, Suav Academy ntawm Sciences, thiab lwm yam. / Stem Cell Reports

Text/Wu Tingyao

"Tsis nco qab koj yog leej twg thiab kuv yog leej twg" tuaj yeem hais tias yog cov tsos mob feem ntau ntawm Alzheimer's disease.Yog vim li cas rau qhov tsis nco qab, lossis tsis tuaj yeem nco qab cov xwm txheej tsis ntev los no, yog cov paj hlwb ua haujlwm ntawm kev paub txog kev txawj ntse tuag me ntsis raws li lub xyoo dhau los, uas ua rau cov neeg laus.kev txawj ntse qibtxuas ntxiv mus degenerate.

Tau ntsib nrog tus kab mob Alzheimer's ntau zuj zus, cov kws tshawb fawb tau ua haujlwm hnyav los kawm txog kev kho mob uas ua tau.Qee tus neeg tsom mus rau qhov ua txhaum cai ua rau cov paj hlwb tuag, sim txo qis kev tsim cov beta-amyloid protein;Lwm tus tau cog lus los txhawb cov paj hlwb rov tsim dua tshiab, vam tias yuav ua rau qhov tsis txaus ntawm cov paj hlwb puas tsuaj, uas yog kab tias lub tswv yim ntawm "ua kom nws yog tias nws ploj lawm."

Nyob rau hauv lub hlwb loj hlob ntawm cov tsiaj nyeg, muaj tseeb ob qhov chaw uas txuas ntxiv tsim cov paj hlwb tshiab, ib qho ntawm cov hippocampal gyrus.Cov paj hlwb uas nws tus kheej proliferating no hu ua "neural progenitor cells".Cov hlwb uas nyuam qhuav yug los ntawm lawv yuav raug muab ntxiv rau hauv qhov qub neural circuits los pab kawm cov txuj ci tshiab thiab tsim kev nco tshiab.

Txawm li cas los xij, nws tuaj yeem pom hauv tib neeg lossis cov nas uas Alzheimer's tus kab mob tuaj yeem cuam tshuam kev loj hlob ntawm neural precursor cells.Niaj hnub no, ntau thiab ntau cov pov thawj qhia tau hais tias txhawb kev loj hlob ntawm neural precursor hlwb tuaj yeem txo qhov kev xav tsis zoo los ntawm Alzheimer's disease thiab tuaj yeem dhau los ua lub tswv yim tsim nyog rau kev kho mob Alzheimer's disease.

Thaum Lub Ib Hlis 2017, kev tshawb fawb tau luam tawm hauv "Stem Cell Reports" los ntawm Tongji University, Shanghai Institutes rau Biological Sciences, Suav Academy ntawm Kev Tshawb Fawb, thiab lwm yam, tau ua pov thawj tias polysaccharides lossis dej rho tawm los ntawmGanoderma lucidum (Reishi Mushroom, Lingzhi) tuaj yeem txo qhov kev puas siab puas ntsws los ntawm Alzheimer's kab mob, txo cov kev tso tawm ntawm amyloid-β (Aβ) hauv lub hlwb, thiab txhawb kev tsim kho ntawm neural precursor hlwb hauv hippocampal gyrus.Cov txheej txheem tom kawg ntawm kev ua haujlwm zoo li yuav muaj feem cuam tshuam nrog kev ua kom lub receptor hu ua FGFR1 ntawm neural precursor cells vim yog kev tswj hwm ntawmGanoderma lucidum.

Alzheimer tus nas nojGanoderma lucidummuaj kev nco zoo dua.

Cov tsiaj sim hauv qhov kev tshawb fawb no siv 5 mus rau 6-hli-laus APP / PS1 transgenic nas - uas yog, kev siv cov noob hloov tshuab hloov pauv cov noob noob neej APP thiab PS1 (uas tuaj yeem ua rau muaj kev cuam tshuam ntxov ntxov ntawm Alzheimer's disease) rau hauv cov nas nyuam qhuav yug los ua kom muaj txiaj ntsig zoo ntawm cov noob.Qhov no yuav ua rau lub hlwb ntawm cov nas pib tsim cov amyloid-β (Aβ) txij thaum yau (tom qab 2 lub hlis ntawm hnub nyoog), thiab thaum lawv loj hlob mus rau 5-6 lub hlis ntawm hnub nyoog, lawv yuav maj mam tsim teeb meem hauv kev paub thiab nco. .

Hauv lwm lo lus, cov nas siv hauv qhov kev sim twb muaj cov tsos mob thawj zaug ntawm Alzheimer's disease.Cov kws tshawb fawb tau noj cov nas Alzheimer nrog GLP (cov ntshiab polysaccharides cais los ntawmGanoderma lucidumspore hmoov nrog ib qho hnyav molecular ntawm 15 kD) ntawm ib hnub koob tshuaj 30 mg / kg (uas yog, 30 mg ib kilogram ntawm lub cev hnyav ib hnub) rau 90 hnub sib law liag.

Tom qab ntawd, cov kws tshawb fawb tau siv sijhawm 12 hnub ntxiv rau kev soj ntsuam lub peev xwm ntawm cov nas hauv Morris dej maze (MWM) thiab muab piv rau cov nas uas muaj Alzheimer's disease uas tsis tau txais kev kho mob thiab nrog cov nas ib txwm muaj.

Cov nas muaj qhov tsis zoo rau dej.Thaum lawv muab tso rau hauv dej, lawv yuav sim nrhiav qhov chaw qhuav kom so.Qhov "Morris Water Maze Test" siv lawv qhov xwm txheej los teeb tsa lub chaw so ntawm qhov chaw ruaj khov hauv lub pas dej loj.Txij li thaum lub platform tau muab zais hauv qab dej, cov nas yuav tsum nrhiav nws tsuas yog los ntawm kev kawm thiab nco ntsoov.Yog li ntawd, cov kws tshawb fawb tuaj yeem txiav txim siab seb cov nas tau txais dumber lossis ntse dua los ntawm lub sijhawm cov nas pom lub platform, qhov deb lawv ua luam dej thiab txoj kev lawv coj mus.

Nws tau pom tias tsis muaj qhov sib txawv tseem ceeb hauv kev ua luam dej nrawm ntawm cov nas hauv txhua pab pawg.Tab sis piv nrog cov nas ib txwm, Alzheimer cov nas uas tsis tau txais kev kho mob yuav tsum tau siv sij hawm ntau dua thiab ua luam dej ntev dua kom pom lub platform raws li txoj kev tsis sib haum xeeb xws li hmoov zoo, qhia tias lawv qhov chaw nco tau poob qis.

Hauv qhov sib piv, Alzheimer's nas noj nrogReishi ncebpolysaccharides los yogGanoderma lucidumdej extract pom lub platform sai dua, thiab ua ntej nrhiav lub platform, lawv tsuas yog taug kev hauv cheeb tsam (quadrant) qhov twg lub platform nyob, zoo li lawv paub qhov kwv yees qhov chaw ntawm lub platform, qhia tias kev puas tsuaj rau lawv lub hlwb tsis tshua muaj mob hnyav.【Daim duab 1, Daim duab 2】

Tsis tas li ntawd, cov kws tshawb fawb kuj tau pom nyob rau hauv lwm qhov kev sim uas rau cov txiv hmab txiv ntoo yoov uas tsim cov amyloid-β (Aβ) ntau hauv lawv lub hlwb (tseem los ntawm kev hloov cov noob los tsim cov qauv kev sim),Ganoderma lucidumdej extract tsis tau tsuas yog txhim kho lub spatial paub thiab nco lub peev xwm ntawm txiv hmab txiv ntoo yoov tab sis kuj yuav ncua lub neej ntawm txiv hmab txiv ntoo yoov.

Cov kws tshawb fawb kuj tau sivGanoderma lucidumdej extract (300mg / kg ib hnub twg) nyob rau hauv cov saum toj no-hais kev sim tsiaj thiab pom tias nws kuj yuav alleviate lub spatial cognitive impairment tshwm sim los ntawm Alzheimer's kab mob ib yam li cov saum toj no hais.Ganoderma lucidumpolysaccharides (GLP).

Siv "Morris Water Maze Test" los ntsuas qhov muaj peev xwm nco tau ntawm nas

[Daim duab 1] Kev ua luam dej ntawm cov nas hauv txhua pab pawg.Qhov xiav yog lub pas dej, qhov dawb yog lub platform txoj hauj lwm, thiab liab yog txoj kev ua luam dej.

[Daim duab 2] Lub sijhawm nruab nrab uas yuav tsum tau muaj rau txhua pab pawg nas kom nrhiav tau lub chaw so rau hnub 7 ntawm Morris dej tshawb nrhiav

(Source/Stem Cell Reports. 2017 Lub Ib Hlis 10; 8(1:84-94.)

Lingzhitxhawb kev loj hlob ntawm neural precursor hlwb hauv hippocampal gyrus.

Tom qab 12-hnub kev tshawb nrhiav dej tshawb nrhiav, cov kws tshawb fawb tau tshuaj xyuas lub hlwb ntawm nas thiab pom tiasGanoderma lucidumpolysaccharides thiabGanoderma lucidumCov dej rho tawm ob qho tib si txhawb kev tsim cov paj hlwb hauv hippocampal gyrus thiab txo cov amyloid-β deposition.

Nws tau raug lees paub ntxiv tias cov paj hlwb uas yug tshiab hauv hippocampus gyrus yog cov neural precursor hlwb.ThiabGanoderma lucidumyog siv tau rau Alzheimer tus kab mob nas.Pub cov me nyuam yau ib txwm nrogGanoderma lucidumpolysaccharides (GLP) ntawm ib hnub koob tshuaj 30 mg / kg rau 14 hnub kuj tseem tuaj yeem txhawb kev loj hlob ntawm neural precursor hlwb hauv hippocampal gyrus.

Cov kev sim hauv vitro kuj tau lees paub tias rau cov neural precursor hlwb cais los ntawm hippocampal gyrus ntawm cov laus laus nas lossis Alzheimer's nas lossis neural precursor hlwb muab los ntawm tib neeg qia hlwb,Ganoderma lucidumpolysaccharides tuaj yeem txhawb nqa cov hlwb ua ntej kom loj hlob, thiab cov hlwb tshiab tau khaws cov yam ntxwv qub ntawm cov hlwb neural precursor, uas yog, lawv tuaj yeem ua kom muaj kev loj hlob thiab rov ua nws tus kheej.

Kev tshuaj xyuas ntxiv qhia tau tiasGanoderma lucidumpolysaccharides (GLP) tuaj yeem txhawb nqa neurogenesis feem ntau vim tias lawv tuaj yeem ntxiv dag zog rau lub receptor hu ua "FGFR1" (tsis yog EGFR receptor) ntawm neural precursor cells, ua rau nws muaj kev cuam tshuam ntau dua rau kev txhawb nqa ntawm "cov paj hlwb loj hlob bFGF", uas xa cov ntaub ntawv ntxiv ntawm "cell. proliferation" mus rau lub neural precursor hlwb, thiab ces ntau cov paj hlwb tshiab tau yug los.

Txij li cov paj hlwb uas nyuam qhuav yug tshiab tuaj yeem txuas ntxiv mus koom nrog cov neural circuits uas twb muaj lawm los ua haujlwm tom qab lawv tsiv mus rau hauv lub paj hlwb uas xav tau, qhov no yuav tsum txo qis ntau yam kev paub tsis zoo los ntawm cov paj hlwb tuag hauv Alzheimer's disease.

Lub luag hauj lwm multifaceted ntawmGanoderma lucidumslows lub pace ntawm tsis nco qab.

Cov kev tshawb fawb saum toj no cia peb pom kev tiv thaiv ntawmGanoderma lucidumntawm paj hlwb.Ntxiv rau nws cov tshuaj tiv thaiv kab mob, tshuaj tiv thaiv oxidant, tshuaj tiv thaiv apoptotic, anti-β-amyloid deposition thiab lwm yam teebmeem uas paub yav dhau los,Ganodermalucidumtuaj yeem txhawb nqa neurogenesis.Rau cov nas Alzheimer uas muaj tib yam caj ces tsis xws luag thiab nyob hauv tib cov tsos mob, qhov no yog vim li cas qhov mob hnyav ntawm tus kab mob no txawv heev ntawm cov neeg noj.Ganoderma lucidumthiab cov uas tsis nojGanoderma lucidum.

Ganoderma lucidumtej zaum yuav tsis tuaj yeem rov qab ua haujlwm nco hauv Alzheimer's cov neeg mob, tab sis nws cov txheej txheem ntawm kev ua haujlwm tuaj yeem ua rau qeeb qeeb ntawm Alzheimer's disease.Tsuav tus neeg mob nco txog nws tus kheej thiab lwm tus mus tas nws lub neej, Alzheimer tus kab mob yuav tsis txaus ntshai.

[Source] Huang S, et al.Polysaccharides los ntawm Ganoderma lucidum Txhawb Kev Paub Txog Kev Ua Haujlwm thiab Neural Progenitor Proliferation hauv nas Model ntawm Alzheimer's Disease.Stem Cell Reports.2017 Jan 10; 8(1:84-94.doi: 10.1016/j.stemcr.2016.12.007.

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Hais txog tus sau/ Ms. Wu Tingyao

Wu Tingyao tau tshaj tawm cov ntaub ntawv Ganoderma thawj zaug txij li xyoo 1999. Nws yog tus sau ntawmKho nrog Ganoderma(tshaj tawm hauv Lub Tsev Kho Mob Tib Neeg Lub Plaub Hlis 2017).

★ Kab lus no tau luam tawm raws li kev tso cai tshwj xeeb ntawm tus sau.★ Cov haujlwm saum toj no tsis tuaj yeem muab luam tawm, txiav tawm lossis siv lwm txoj hauv kev yam tsis muaj kev tso cai los ntawm tus sau.★ Rau kev ua txhaum ntawm cov lus hais saum toj no, tus sau yuav ua raws li cov luag haujlwm raug cai.★ Cov ntawv qub ntawm kab lus no tau sau ua lus Suav los ntawm Wu Tingyao thiab txhais ua lus Askiv los ntawm Alfred Liu.Yog tias muaj qhov tsis sib xws ntawm kev txhais lus (English) thiab tus thawj (Suav), tus thawj Suav yuav yeej.Yog tias cov nyeem muaj lus nug, thov hu rau tus thawj kws sau ntawv, Ms. Wu Tingyao.