I-Chemotherapylimazas isibindi nezinso ngenkathiLingzhi (ngiphinde ngibizweI-Ganoderma lucidum noma Reishi mushroom) ivikela isibindi nezinso.

AngakwaziI-Ganoderma lucidum ukumelana nokulimala kwesibindi nezinso okubangelwa ukwelapha ngamakhemikhali?

Ithimba elakhiwe uSolwazi u-Hanan M Hassan ovela e-Faculty of Pharmacy yase-Delta University for Science & Technology e-Egypt kanye noSolwazi Yasmen F Mahran we-Faculty of Pharmacy yase-Ain Shams University e-Egypt basebenzise i-cisplatin, umuthi ovamile wokwelapha ngamakhemikhali, ukuze bahlole. okungenzeka ukuthiI-Ganoderma lucidum ekuvikeleni amaseli esibindi nezinso ekulimaleni kwe-cisplatin.

Imiphumela yabo yocwaningo ihlukaniswe yaba izihloko ezimbili: eyodwa ivikela isibindi kanti enye ivikela izinso.Zishicilelwe ku-“Drug Design, Development and Therapy” kanye “ne-Oxidative Medicine and Cellular Longevity” ngoJuni nangoJulayi 2020, ngokulandelana.

I-anti-oxidant, anti-inflammatory kanye ne-anti-apoptotic imiphumela yeI-Ganoderma lucidum ngokusobala ingavimba umonakalo omningi we-oxidative, umonakalo ovuvukalayo kanye ne-cell apoptosis ebangelwa i-cisplatin, futhi lokho kuvikela kusebenza kumaseli esibindi noma amaseli ezinso.Lokhu akugqamisi nje kuphela inani lomuthi elikabiliI-Ganoderma lucidum kodwa futhi inikeza indlela yokuvikela eyisizayo yokwelashwa ngamakhemikhali omdlavuza.

Ukuze ugweme ukwenza lesi sihloko sibe side kakhulu, umbhali uzokwethula indima yeI-Ganoderma lucidum kulesi sici ezingxenyeni ezimbili ngethemba lokuthi le datha esekelwe ngokwesayensi kanye nobufakazi kuzoletha ukuzethemba okwengeziwe kubangane abafuna ukunciphisa imiphumela emibi yokwelashwa ngamakhemikhali.

Ingxenye 1I-Ganoderma lucidum ivikela isibindi ngokumelene ne-cisplatin hepatotoxicity

I-rAbacwaningi baqhathanisa umehlukosphakathi kokusebenzisa nokungasebenzisiI-Ganoderma lucidumngesikhathi sokwelashwa kwe-cisplatinemaqenjini ayisithupha amagundane anempilo kanye nomehluko ekuvikelekeni ekulimaleni kwesibindi okuhlukileI-Ganoderma lucidum izindlela zokuphatha.Kunjalo:

◆Iqembu Lokulawula (Qhubeka): iqembu elingakutholi ukwelashwa;

◆I-Ganoderma lucidumIqembu(GL): iqembu elingajovwe nge-cisplatin kodwa liyadlaI-Ganoderma lucidum nsuku zonke;

◆I-Cisplatin Group (CP): iqembu elijovwe nge-cisplatin kuphela kodwa elingadliI-Ganoderma lucidum;

◆Iqembu lansuku zonke (Nsuku zonke): iqembu elijovwe cisplatinfuthi uyadlaI-Ganoderma Lucidum nsuku zonke;

◆Njalo Ngelinye Iqembu Lezinsuku (EOD): iqembu elijovwe cisplatinfuthi uyadlaI-Ganoderma lucidum zonke izinsuku;

◆Iqembu le-Intraperitoneal (ip): iqembu elijovwe cisplatinfuthi uthola ii-traperitonealiukwenziwa kweGi-anoderma lucidum.

Bonke labo abathola i-cisplatin bajovwa nge-intraperitoneally nge-12 mg/kg yeCisplatinngosuku lokuqala lokuhlola ukuqala ukulimala kwesibindi okukhulu;labo abathole umjovo we-intraperitoneal weI-Ganoderma lucidum zajovwa kanye ngosuku lwesibili nolwesithupha lokuhlolwa.

II-Ganoderma lucidum esetshenziswe ocwaningweni iqukethe izithako ezisebenzayo ezifana nama-triterpenes, ama-sterol, ama-polysaccharides, ama-polyphenols nama-flavonoid.II-Ganoderma lucidum enikezwa ekuhloleni kwezilwane, noma ngabe ithathwa ngomlomo noma ngomjovo, ibalwa ngethamo lansuku zonke lama-500 mg/kg.

(1)I-Ganoderma lucidum kunciphisa ukulimala kwe-hepatocellular

Ngemuva kwezinsuku eziyi-10, kungabonakala ukuthi i-cisplatin izonyusa inkomba ye-hepatitis kanye nezinga le-bilirubin eliphelele ku-serum yegundane.Lezi zonke izimpawu zokulimala kwe-hepatocellular.Kodwa umaI-Ganoderma lucidum uhilelekile ngesikhathi esifanayo, inani elikhulayo lingancishiswa kakhulu (Umfanekiso 1).

Umthombo Wedatha/Izidakamizwa Des Devel Ther.2020;14:2335-2353.

Umfanekiso 1 Imiphumela ye-cisplatin neI-Ganoderma lucidum ezinkomba zokulimala kwesibindi

Faka ingxenye yezicubu zesibindi ngaphansi kwesibonakhulu, futhi uyabona ukuthi i-cisplatin ingabangela ukuminyana kwesibindi (igazi okufanele libuyele enhliziyweni livalekile futhi limile emithanjeni ye-hepatic), ukuwohloka kwamaseli (kuvela ama-vacuoles, okuwushintsho lokuqala ukulimala kwamaselula), i-apoptosis kanye ne-necrosis, kodwa lezi zimo zingancishiswa ngokusebenzisaI-Ganoderma lucidum.

Umfanekiso 2 Imiphumela ye-cisplatin neI-Ganoderma lucidum kuma-hepatocytes

(2)I-Ganoderma lucidum kuthuthukisa umthamo we-antioxidant wamaseli esibindi

Lesi sihloko siphinde siqhathanise umonakalo we-oxidative obhekene neqembu ngalinye lezicubu zesibindi.Kunezinkomba ezimbili zokubuka: i-MDA (malondialdehyde), umkhiqizo owakhiwe ngemva kokucekelwa phansi kolwelwesi lwamaseli ngama-radicals mahhala, kanye no-H₂O₂ (i-hydrogen peroxide), umkhiqizo ophakathi nendawo owakhiwe ngemva kokuguqulwa kwama-radicals mahhala ngama-enzyme e-antioxidant.

Yomibili le mikhiqizo inezici ze-oxidative zama-radicals mahhala futhi kufanele iphinde ilashwe ngaphambi kokuthi "isuswe ubuthi" ngempela, ukuze inani layo lingasitshela ngomonakalo we-oxidative izicubu zesibindi. “Unayo wahlupheka” futhi “uzohlupheka”.

Ngokusobala, i-cisplatin izodala umonakalo omkhulu we-oxidative ezicutshini zesibindi, kodwa umaI-Ganoderma lucidum kuhilelekile Ekwelapheni ngesikhathi esifanayo, umonakalo onjalo ungancishiswa (Umfanekiso 3).

Ngoba izinguquko ekugxilweni kwama-enzyme e-antioxidant (i-SOD ne-GSH) ezicutshini zesibindi zeqembu ngalinye kanye nezinguquko ezinkomba zokulimala kwe-oxidative zibonise umkhuba ohluke ngokuphelele., kungacatshangwa ukuthiI-Ganoderma lucidumizokwandisa umthamo we-antioxidant wezicubu zesibindi futhi inciphise umonakalo "ngokwandisa ama-enzyme e-antioxidant".

Umfanekiso3 Imiphumela ye-cisplatin kanyeI-Ganoderma lucidum ekulimaleni kwe-oxidative kwezicubu zesibindi

(3)I-Ganoderma lucidum kuthuthukisa ikhono lokulwa nokuvuvukala kwamaseli esibindi

I-Cisplatin isongela ukuphila kwamangqamuzana ngokulimaza i-DNA kanye nokuyenga inani elikhulu lama-radicals mahhala;amaseli angaphansi kwengcindezi azovula inkinobho enkulu i-NF-kB elawula ukusabela kokuvuvukala, ibangele amaseli ukuthi ahlanganise futhi akhulule isici se-tumor necrosis (TNF-α) namanye ama-cytokines ukuze enze kusebenze igagasi lokuqala lokusabela kokuvuvukala futhi akhalise i-alamu yokugonywa komzimba.

Ngokushesha ngemva kwalokho, lawo mangqamuzana abulawa ukulimala kwe-oxidative noma ukuvuvukala azokhulula enye i-cytokine, i-HMGB-1, ukuze isebenze amangqamuzana omzimba amaningi, okubangela amagagasi okuvuvukala.

Ukuvuvukala okuqhubekayo ngeke nje kuqinise umonakalo we-oxidative kodwa futhi kuqhubekisele amangqamuzana amaningi ekufeni, futhi kubangele ngisho nezicubu zesibindi ukuthi kancane kancane zithuthukise i-fibrosis ngesikhathi sokuvuvukala okuphindaphindiwe nokulungiswa.

Ngenhlanhla, njengoba njeI-Ganoderma lucidum kunganciphisa umonakalo we-oxidative obangelwa yi-cisplatin, ukuhlolwa kwezilwane nakho kwaqinisekisa ukuthi ukusetshenziswa okuhlangene kwe-cisplatin kanyeI-Ganoderma lucidum ingavimbela ukusebenza kwe-inflammation switch i-NF-kB, inciphise i-TNF ekhuthaza ukuvuvukala-akanye ne-HMGB-1, futhi andisei-anti-inflammatory cytokine IL-10ezicutshini zesibindi ngesikhathi esifanayo (Umfanekiso 4).

Ihlanganiswe ndawonye, ​​le miphumela ayivimbeli ukuvuvukala kuphela kodwa futhi inciphisa ukufakwa kwe-collagen futhi ivimbele ukuqhubeka kwesibindi se-fibrosis (Umfanekiso 5).

Umthombo Wedatha/Izidakamizwa Des Devel Ther.2020;14: 2335-2353.

Umfanekiso 4 Imiphumela ye-cisplatin neI-Ganoderma lucidum ngokuvuvukala kwezicubu zesibindi

Umthombo Wedatha/Izidakamizwa Des Devel Ther.2020;14: 2335-2353.

Umfanekiso 5 Imiphumela ye-cisplatin ne-Ganoderma lucidum ku-liver fibrosis

(4)I-Ganoderma lucidum kuthuthukisa ikhono le-anti-apoptotic lamaseli esibindi

Kungakhathaliseki ukuthi ngokulimala kwe-oxidative noma ukulimala kokuvuvukala, i-cisplatin izogcina isebenze indlela ye-“apoptosis” futhi iphoqelele amaseli esibindi ukuthi afe.

Ngamanye amazwi, uma amangqamuzana esibindi engakwazi ukubamba umugqa wokugcina wokuzivikela, azoba namathuba amaningi okusinda futhi anciphise ubunzima bokulimala kwesibindi.

Kunama-molecule amaningi amaprotheni alawula i-apoptosis.Phakathi kwabo, abamele kakhulu yilezi: i-p53, engakhuthaza i-apoptosis, i-Bcl-2, engavimbela i-apoptosis, ne-caspase-3, eyenza i-apoptosis ngomzuzu wokugcina.

Ngokusho kwabacwaningi'ukuhlaziywa kwezicubu zesibindi zezilwane zokuhlola eqenjini ngalinye,I-Ganoderma lucidum ayikwazi nje ukukhuthaza inkulumo ye-Bcl-2 kodwa futhi ivimbele inkulumo ye-p53 ne-caspase-3, enganikeza amandla anamandla e-anti-apoptotic kumaseli esibindi.

(5) Ama-Ganoderic acids adlala indima ebalulekile yokulwa nokuvuvukala

Kusukela ku-anti-oxidation, anti-inflammation, anti-apoptosis ekusebenzeni kwangempela kokunciphisa ukulimala kwesibindi, abacwaningi baye bahlanganisa indlelaI-Ganoderma lucidum ekuvimbeleni i-cisplatin hepatotoxicity kumdwebo olandelayo ukuze uthole ireferensi yakho.

Umthombo Wedatha/Izidakamizwa Des Devel Ther.2020;14: 2335-2353.

Umfanekiso 6 Indlela ye-Ganoderma lucidum ekuvimbeleni ubuthi besibindi be-cisplatin

Ekupheleni kwalolu cwaningo, ukuhlaziya“isistimu yokulingisa i-molecular docking”wathola ukuthi okungenani 14 ganoderic acid in the triterpenes ofI-Ganoderma lucidum (njengoba kukhonjisiwe kuthebula elingezansi) ingabophezela ngokuqondile nangempumelelo kukhiye we-cytokine HMGB-1, ngaleyo ndlela yenze umsebenzi we-pro-inflammatory we-HMGB-1 ungasebenzi.

Njengoba ukulwa nokuvuvukala kungenye yezindlela ezibalulekile zeI-Ganoderma lucidum ukunciphisa i-hepatotoxicity eyenziwe yi-Cisplatin,“ingcebo eGanoderic i-asidi”isibe yingxenye yenkomba yeI-Ganoderma lucidum ukuvikela isibindi.

Hlobo luni lweI-Ganoderma lucidum isithakos ingaqukatha i-Ganoder eningi kangakaic i-asidis?Ngokocwaningo lwangaphambilini, kuyaziwa ukuthi bakhona ikakhulukazi “I-Ganoderma lucidum izitheloing ukukhishwa kotshwala bomzimba”.

Kuyafaneleka ukubalula ukuthi amagundane in theI-Ganoderma lucidum iqembu elalidla kuphelaI-Ganoderma lucidum acishe afane namagundane e-ukulawula iqembu emiphumeleni yokuhlola eshiwo ngenhla, ebonisa lokhoI-Ganoderma lucidum iphephe kakhulu ukuthi isetshenziswe.

Ngaphezu kwalokho, indlela yokusebenzisaI-Ganoderma lucidum nayo ibaluleke kakhulu.Uma uzimisele uku-revawu ishadi eliboniswe kulesi sihloko, akunzima ukuthola ukuthi “EkakhuluDay Iqembu” inomphumela omuhle kakhulu.

Empeleni, EkakhuluDay Iqembu has umphumela omuhle kakhulu ekunciphiseni i-hepaticfuthi ubuthi bezinso be-cisplatin ekuhlolweni kwezilwane,okusho ukuthi ahluket kusuka okunyeGi-anoderma lucidum amaqembu.

Yikuphi ukubonakaliswa okuqondile kwemiphumela emihle eshiwo ngenhla?Hlala ubukele “Ingxenye 2I-Ganoderma lucidum ivikela izinso vs. Cisplatin nephrotoxicity”.

[Umthombo Wedatha]

1.Hanan M Hassan, et al.Ukucindezelwa Kokulimala Kwesibindi Okubangelwa I-Cisplatin Kumagundane Nge-Alarmin High-Mobility Group Box-1 Pathway byI-Ganoderma lucidum: Ucwaningo Lwethiyori Nokuhlola.Izidakamizwa Des Devel Ther.2020;14:2335-2353.

2.Yasmen F Mahran, et al.I-Ganoderma lucidumIvimbela I-Cisplatin-Induced Nephrotoxicity Ngokuvinjelwa Kwe-Epidermal Growth Factor Receptor Signaling kanye ne-Autophagy-Mediated Apoptosis.I-Oxid Med Cell Longev.2020. doi: 10.1155/2020/4932587.

PHELA

Mayelana nombhali/ Nks. Wu Tingyao

U-Wu Tingyao ubelokhu ebika mathuphaI-Ganoderma lucidumulwazi kusukela ngo-1999. Ungumbhali weUkwelapha nge-Ganoderma(ishicilelwe ku-People's Medical Publishing House ngo-Ephreli 2017).

★ Lesi sihloko sishicilelwe ngaphansi kokugunyazwa okukhethekile kombhali, futhi ubunikazi bungeka-GANOHERB ★ Le misebenzi engenhla ayinakuphinda ikhiqizwe, ikhishwe noma isetshenziswe ngezinye izindlela ngaphandle kokugunyazwa kwe-GanoHerb ★ Uma imisebenzi igunyaziwe ukuthi isetshenziswe, kufanele isetshenziswe ngaphakathi kobubanzi bokugunyazwa futhi ibonise umthombo: GanoHerb ★ Ukwephulwa kwesitatimende esingenhla, i-GanoHerb izoqhuba izibopho zayo ezingokomthetho ezihlobene ★ Umbhalo wokuqala wale ndatshana ubhalwe ngesiShayina nguWu Tingyao futhi wahunyushelwa esiNgisini ngu-Alfred Liu.Uma kukhona ukungqubuzana phakathi kokuhumusha (isiNgisi) kanye nokwangempela (isiShayina), isiShayina sangempela siyonqoba.Uma abafundi benemibuzo, sicela uthinte umbhali wokuqala, uNksz. Wu Tingyao.

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