I-“Anti-Cancer Agents in Medicinal Chemistry” ekhishwe ngokusemthethweni ngoFebhuwari 2020 yashicilela umphumela wocwaningo wethimba likaSolwazi Li Peng waseSikoleni Sekhemisi saseFujian Medical University.Ucwaningo luqinisekise ngokuhlolwa kweseli nezilwane okuvela kuzo i-neutral triterpenesI-Ganoderma lucidumingavimbela ngokuphawulekayo ukukhula komdlavuza we-colorectal, futhi indlela yawo yokusebenza ihlobene "nokugqugquzela i-apoptosis yamangqamuzana omdlavuza."

Isamba se-Triterpenes = I-Neutral Triterpenes + I-Acidic Triterpenes

Kusukela ekutholweni kokuqala kweI-Ganoderma lucidumtriterpenes ngo-1982, ososayensi abanikezanga kuphela izincazelo zesayensi zokuthi “kunganiI-Ganoderma lucidumimizimba ethela izithelo iyababa kakhulu” kodwa iphinde inikeze nezinkomba ngaphandle kwama-polysaccharides ocwaningweni lokuthi “KunganiI-Ganoderma lucidumi-anti-tumor".

I-Ganoderma lucidumI-triterpenes iyibizo elihlangene, elibhekisela kuzithako ezisebenzayo kuI-Ganoderma lucidumngesakhiwo se-terpene.Ngokusho kwesakhiwo sabo samakhemikhali, zingahlukaniswa ngamaqembu amabili: iqembu elilodwa "liyi-acidic triterpenes" kuhlanganise nama-anoderic acid ahlukahlukene (ingxenye ye-acidic triterpene, i-ATF), kanti elinye iqembu "liyi-neutral triterpenes" kuhlanganise nama-ganoderiols ahlukahlukene ("ingxenye ye-neutral triterpene ", NTF).Lapho la maqembu amabili ama-triterpenes ehlanganiswa, abizwa ngokuthi ama-triterpenes aphelele.

Nakuba kunobufakazi obuningi besayensi bemiphumela ephikisana ne-tumor yeI-Ganoderma lucidumingqikithi ye-triterpenes kanye ne-acidic triterpenes, kunezifundo ezimbalwa ngendima yeI-Ganoderma lucidumama-triterpenes angathathi hlangothi kulokhu.Ngakho-ke, ithimba likaProfesa Li Peng lagxila kule ngxenye.

UkusebenzisaI-Ganoderma lucidumimizimba ethela izithelo (ehlinzekwe yi-Fujian Xianzhilou Biological Science and Technology Co., Ltd.) njengento yokuhlola, ithimba laqala lakhipha ingqikithi yama-triterpenesI-Ganoderma lucidumkusukela emizimbeni enezithelo zeI-Ganoderma lucidumnge-ethanol, yase iphinda ihlukanise i-neutral triterpenes kanye ne-acidic triterpenes ukuze ihlole umphumela wayo ovimbelayo kumdlavuza we-colorectal.

Ukuhlolwa kweseli: umphumela wokulwa nomdlavuza we-neutral triterpenes＞umphumela wokulwa nomdlavuza we-acidic triterpenes

Abacwaningi bahlakulela ngokwehlukanaI-Ganoderma lucidumI-neutral triterpenes kanye ne-acidic triterpenes enezinhlobo ezintathu ezahlukene zamaseli omdlavuza we-colorectal womuntu amahora angama-48.Kokuphelele,I-Ganoderma lucidumama-triterpenes angathathi hlangothi abe nemiphumela engcono kakhulu yokuvimbela ukukhula kwamangqamuzana omdlavuza (ukwanda) kunokubaI-Ganoderma lucidumi-acidic triterpenes (Umfanekiso 1).

Ukuhlolwa kwezilwane:I-Ganoderma lucidumI-neutral triterpenes ivimbela ngempumelelo ukukhula kwesimila

Abacwaningi baphinde bahlola imiphumela ye-anti-tumor yeI-Ganoderma lucidumI-neutral triterpenes ku-vivo ngokuhlolwa kwezilwane: Okokuqala, umugqa weseli lomdlavuza we-colorectal SW620 onekhono le-lymphatic metastasis wafakwa ngaphansi kwesikhumba samagundane anqunu (amagundane angenawo amasosha omzimba).Ngemuva kokuvela kwesimila, ama-250 mg/kg noma ama-500 mg/kg weI-Ganoderma lucidumama-triterpenes angathathi hlangothi anikezwa ngomlomo kumagundane nsuku zonke.

Ngemva kwezinsuku ezingu-13 zokuhlolwa, kwatholakala ukuthi ukungenelela kweI-Ganoderma lucidumi-neutral triterpenes ingenza amathumba akhule kancane futhi abe mancane, futhi umphumela wawo ovimbelayo uqhathaniswa nalowo womuthi we-chemotherapy 5-Fu (20 mg/kg intraperitoneal injection ngosuku), kodwa awubangeli ukuncipha okukhulu kwesisindo njengo-5-Fu ( Umfanekiso 2-5).

Izithako ezisebenzayo: okungenani izinhlobo eziyisishiyagalolunye zama-triterpenes

Njengoba kushiwo ngaphambili esihlokweni,I-Ganoderma lucidumI-neutral triterpenes nayo iyingxube, equkethe izinhlobonhlobo zama-triterpenes ahlukene.Ngokokuhlaziya komcwaningi, ama-triterpenes angathathi hlangothi ashiwo ngenhla weI-Ganoderma lucidum, enomphumela ovimbelayo kumdlavuza we-colorectal, iqukethe okungenani izinhlobo eziyisishiyagalolunye zama-triterpenes (Umfanekiso 6).

Indlela yokwenza: ukukhuthaza i-apoptosis yamaseli omdlavuza

Uma lawa ma-triterpenes ayisishiyagalolunye ekhuliswa ngokuhlukana nomugqa weseli lomdlavuza we-colorectal SW620 in vitro, azotholakala enamandla noma amancane okubulala amaseli omdlavuza.

Abacwaningi baphinde bahlola i-ganoderma triterpene (ganodermanondiol) emele kakhulu futhi bathola ukuthi ingaqalisa indlela ye-apoptosis yamangqamuzana omdlavuza futhi isunduze amangqamuzana omdlavuza isuka e-altare elingapheli iye kwalasha wokufa.Ukugxila kwayo okusebenzayo (ukuvinjwa kwesigamu samangqamuzana omdlavuza) kungu-11.17 μg/mL.

Lokhu kugxila akunabungozi kumaseli ajwayelekile (umugqa weseli wegundane we-embryonic fibroblast NIH3T3), futhi ukugxilisa ingqondo kufanele kunyuswe kube ngaphezu kuka-80 μg/mL ukuze kube nomthelela obalulekile (ukuvimbela ukusinda kwengxenye yemigqa yamaseli e-embryonic yegundane).Lokhu kusho ukuthi i-ganodermanondiol ingakwazi ukuhlukanisa amangqamuzana omdlavuza kumaseli avamile futhi inikeze "ukwelashwa" okuhlukile, okuhluke ngokuphelele kumphumela "wokubulala" wezidakamizwa ze-chemotherapeutic kumaseli amahle namabi.

Ukuthi leziI-Ganoderma lucidumI-neutral triterpenes iqala indlela ye-apoptosis yamaseli omdlavuza ifinyelelwa ngokulawula i-mitochondria kumaseli omdlavuza ikufanele ukuphenywa ngokuqhubekayo ngokusho kwabacwaningi.

[Izithenjwa]

Li P, et al.I-Anti-Cancer Effects ye-Neutral Triterpene Fraction evela ku-Ganoderma lucidum kanye Nezingxenye zayo Ezisebenzayo Kumaseli Womdlavuza We-Colorectal Womuntu we-SW620.Ama-Anticancer Agents Med Chem.2020;20(2): 237-244.doi: 10.2174/1871520619666191015102442.

PHELA

Mayelana nombhali/ Nks. Wu Tingyao
U-Wu Tingyao ubelokhu ebika nge-Ganoderma yokuqala ulwazi kusukela ngo-1999. Ungumbhali weUkwelapha nge-Ganoderma(ishicilelwe ku-People's Medical Publishing House ngo-Ephreli 2017).

★ Lesi sihloko sishicilelwe ngaphansi kokugunyazwa okukhethekile kombhali, futhi ubunikazi bungobe-GANOHERB.★ Imisebenzi engenhla ayikwazi ukukhiqizwa kabusha, ikhishwe noma isetshenziswe ngezinye izindlela ngaphandle kokugunyazwa kwe-GanoHerb.★ Uma imisebenzi igunyaziwe ukuthi isetshenziswe, kufanele isetshenziswe ngaphakathi kobubanzi bokugunyazwa futhi ibonise umthombo: GanoHerb.★ Kunoma yikuphi ukwephulwa kwesitatimende esingenhla, i-GanoHerb izolandela izibopho ezingokomthetho ezihlobene.★ Umbhalo wokuqala walesi sihloko ubhalwe ngesiShayina nguWu Tingyao futhi wahunyushelwa esiNgisini ngu-Alfred Liu.Uma kukhona ukungqubuzana phakathi kokuhumusha (isiNgisi) kanye nokwangempela (isiShayina), isiShayina sangempela siyonqoba.Uma abafundi benemibuzo, sicela uthinte umbhali wokuqala, uNksz. Wu Tingyao.