"Ii-Anti-Cancer Agents kwi-Medicinal Chemistry" ekhutshwe ngokusesikweni ngoFebruwari 2020 yapapasha isiphumo sophando seqela likaNjingalwazi uLi Peng ovela kwiSikolo seKhemesti saseFujian Medical University.Uphando luqinisekisiwe ngeseli kunye novavanyo lwezilwanyana ukuba i-triterpenes engathathi hlangothi ukusukaI-Ganoderma luciduminokuthintela ngokubonakalayo ukukhula komhlaza wesisu, kwaye indlela yokusebenza kwayo inxulumene "nokukhuthaza i-apoptosis yeeseli zomhlaza."

Iyonke i-Triterpenes = i-Neutral Triterpenes + i-Acidic Triterpenes

Ukususela ekufumaneni kokuqala kweI-Ganoderma lucidumtriterpenes ngo-1982, izazinzulu azinikanga kuphela iingcaciso zenzululwazi “kutheniI-Ganoderma lucidumimizimba eneziqhamo ikrakra kakhulu” kodwa ikwabonelela ngemikhondo ngaphandle kwepolysaccharides kuphononongo lokuba “KutheniI-Ganoderma lucidumichasene nethumba ".

I-Ganoderma lucidumI-triterpenes sisibizo esidibeneyo, esibhekisa kwizithako ezisebenzayo kwiI-Ganoderma lucidumngesakhiwo se-terpene.Ngokwesakhiwo sabo semichiza, zinokwahlulwa zibe ngamaqela amabini: elinye iqela "li-acidic triterpenes" kubandakanywa ne-ganoderic acids (iqhezu le-acidic triterpene, i-ATF), kunye nelinye iqela "li-triterpenes elingathathi hlangothi" kuquka i-ganoderiols ("i-neutral triterpene fraction". ", NTF).Xa la maqela mabini e-triterpenes edityanisiwe, abizwa ngokuba zii-triterpenes ezipheleleyo.

Nangona kukho ubungqina obuninzi besayensi malunga neziphumo ezichasene ne-tumorI-Ganoderma lucidumzizonke triterpenes kunye triterpenes acidic, kukho izifundo ezimbalwa indimaI-Ganoderma lucidumii-triterpenes ezingathathi hlangothi kulo mba.Ngoko ke, iqela likaNjingalwazi uLi Peng ligxile kule nxalenye.

UkusebenzisaI-Ganoderma lucidumimizimba eneziqhamo (ebonelelwe nguFujian Xianzhilou Biological Science and Technology Co., Ltd.) njengesixhobo sovavanyo, iqela laqala lakhupha iitriterpenes zizonke zeI-Ganoderma lucidumukusuka kwimizimba eneziqhamo zeI-Ganoderma lucidumkunye ne-ethanol, kwaye emva koko yahlula i-triterpenes engathathi hlangothi kunye ne-acidic triterpenes ukuhlola umphumo wabo wokuthintela kumhlaza we-colorectal.

Uvavanyo lweseli: impembelelo yokuchasana nomhlaza ye-triterpenes engathathi hlangothi＞impembelelo yokuchasana nomhlaza ye-acidic triterpenes

Abaphandi baphucula ngokwahlukeneyoI-Ganoderma lucidumi-triterpenes engathathi hlangothi kunye ne-acidic triterpenes eneentlobo ezintathu ezahlukeneyo zeeseli zomhlaza webala lomntu kwiiyure ezingama-48.Emngxunyeni,I-Ganoderma lucidumI-triterpenes engathathi hlangothi yayineziphumo ezingcono kakhulu zokuthintela ukukhula kweeseli zomhlaza (ukwanda) kunokubaI-Ganoderma lucidumi-acidic triterpenes (Umfanekiso 1).

Umfuniselo wezilwanyana:I-Ganoderma lucidumiitriterpenes ezingathathi hlangothi zithintela ngokufanelekileyo ukukhula kwethumba

Abaphandi baphinda bavavanya iziphumo ezichasene ne-tumorI-Ganoderma lucidumI-triterpenes engathathi hlangothi kwi-vivo ngovavanyo lwezilwanyana: Okokuqala, umgca weseli yomhlaza we-colorectal we-SW620 enobuchule be-lymphatic metastasis yafakwa phantsi kwesikhumba seempuku ezinqunu (iimpuku ezingenamajoni omzimba).Emva kokuba ithumba liye lavela, 250 mg / kg okanye 500 mg / kgI-Ganoderma lucidumii-triterpenes ezingathathi hlangothi zinikwa ngomlomo kwiimpuku yonke imihla.

Emva kweentsuku ezili-13 zovavanyo, kwafunyaniswa ukuba ungenelelo lweI-Ganoderma lucidumI-triterpenes engathathi hlangothi inokwenza ukuba amathumba akhule kancinci kwaye abencinci, kwaye isiphumo sawo sokuthintela sinokuthelekiswa neyeza le-chemotherapy ye-5-Fu (i-20 mg / kg ye-intraperitoneal injection ngosuku), kodwa ayibangeli ukwehla kobunzima obunzulu njenge-5-Fu ( Umfanekiso 2-5).

Izithako ezisebenzayo: ubuncinane iintlobo ezilithoba zeetriterpenes

Njengoko kuchaziwe ngaphambili kwinqaku,I-Ganoderma lucidumii-triterpenes ezingathathi hlangothi nazo zingumxube, oqulethe iindidi ezahlukeneyo ze-triterpenes.Ngokohlalutyo lomphandi, i-triterpenes engathathi hlangothi ekhankanywe ngasentla yeI-Ganoderma lucidum, enempembelelo yokuthintela umhlaza we-colorectal, iqulethe ubuncinane iintlobo ezilithoba ze-triterpenes (Umfanekiso 6).

Indlela yokwenza: ukukhuthaza i-apoptosis yeeseli zomhlaza

Ukuba ezi zisithoba ze-triterpenes zikhuliswe ngokwahlukeneyo kunye nomgca weseli yomhlaza we-colorectal SW620 kwi-vitro, ziya kufunyanwa zikwazi ukubulala iiseli zomhlaza.

Abaphandi baphinda baphonononga eyona ganoderma triterpene imele (ganodermanondiol) kwaye bafumanisa ukuba inokuqalisa indlela ye-apoptosis yeeseli zomhlaza kwaye ityhale iiseli zomhlaza ukusuka kwisibingelelo esingapheliyo ukuya enzonzobileni yokufa.Ukugxininiswa kwayo okusebenzayo (ukuvinjelwa kwesiqingatha seeseli zomhlaza) ngu-11.17 μg / mL.

Olu gxininiso alunabungozi kwiiseli eziqhelekileyo (i-mouse embryonic fibroblast cell line NIH3T3), kwaye ugxininiso kufuneka lunyuswe ngaphezu kwe-80 μg / mL ukuze lube nempembelelo ebalulekileyo (ukuthintela ukusinda kwesiqingatha semigca yeseli ye-mouse embryonic).Oku kuthetha ukuba i-ganodermanondiol inokwahlula iiseli zomhlaza kwiiseli eziqhelekileyo kwaye inike "unyango" olwahlukileyo, oluhluke ngokupheleleyo kwisiphumo "sokubulala" samachiza e-chemotherapeutic kwiiseli ezilungileyo nezimbi.

Nokuba eziI-Ganoderma lucidumI-triterpenes engathathi hlangothi iqalisa indlela ye-apoptosis yeeseli zomhlaza iphunyezwa ngokulawula i-mitochondria kwiiseli zomhlaza ilufanele uphando olongezelelweyo ngokutsho kwabaphandi.

[Iimbekiselo]

ULi P, nabanye.Iimpembelelo ezichasene noMhlaza weNgxelo ye-Triterpene engathathi hlangothi ukusuka kwi-Ganoderma lucidum kunye neMiba eSebenzayo kwi-SW620 yeeSeli zoMhlaza woMbala woMntu.Iiarhente zeAnticancer Med Chem.2020;20(2): 237-244.doi: 10.2174/1871520619666191015102442.

ISIPHELO

Malunga nombhali/ Nksz. Wu Tingyao
UWu Tingyao unike ingxelo ngeGanoderma yokuqala ulwazi ukususela ngo-1999. Ungumbhali weUkunyanga ngeGanoderma(ipapashwe kwiNdlu yePeople's Medical Publishing House ngo-Epreli 2017).

★ Eli nqaku lipapashwe phantsi kogunyaziso olulodwa lombhali, kwaye ubunini bobuka-GANOHERB.★ Le misebenzi ingentla ayinakuphinda iveliswe, ikhutshwe okanye isetyenziswe ngezinye iindlela ngaphandle kokugunyaziswa kweGanoHerb.★ Ukuba imisebenzi igunyazisiwe ukuba isetyenziswe, kufuneka isetyenziswe ngaphakathi kwendawo yogunyaziso kwaye ibonise umthombo: GanoHerb.★ Nakuphi na ukuphulwa kwengxelo engentla, i-GanoHerb iya kulandela uxanduva olusemthethweni olunxulumeneyo.★ Umbhalo wokuqala weli nqaku ubhalwe ngesiTshayina nguWu Tingyao waza waguqulelwa kwisiNgesi nguAlfred Liu.Ukuba kukho nakuphi na ukungangqinelani phakathi kwenguqulelo (isiNgesi) kunye neyoqobo (isiTshayina), isiTshayina sokuqala siya koyisa.Ukuba abafundi banayo nayiphi na imibuzo, nceda uqhagamshelane nombhali wokuqala, uNks. Wu Tingyao.